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Distinct DNA repair pathways cause genomic instability at alternative DNA structures

Author

Listed:
  • Jennifer A. McKinney

    (The University of Texas at Austin, Dell Pediatric Research Institute)

  • Guliang Wang

    (The University of Texas at Austin, Dell Pediatric Research Institute)

  • Anirban Mukherjee

    (The University of Texas at Austin, Dell Pediatric Research Institute)

  • Laura Christensen

    (The University of Texas at Austin, Dell Pediatric Research Institute)

  • Sai H. Sankara Subramanian

    (The University of Texas at Austin, Dell Pediatric Research Institute)

  • Junhua Zhao

    (The University of Texas at Austin, Dell Pediatric Research Institute)

  • Karen M. Vasquez

    (The University of Texas at Austin, Dell Pediatric Research Institute)

Abstract

Alternative DNA structure-forming sequences can stimulate mutagenesis and are enriched at mutation hotspots in human cancer genomes, implicating them in disease etiology. However, the mechanisms involved are not well characterized. Here, we discover that Z-DNA is mutagenic in yeast as well as human cells, and that the nucleotide excision repair complex, Rad10-Rad1(ERCC1-XPF), and the mismatch repair complex, Msh2-Msh3, are required for Z-DNA-induced genetic instability in yeast and human cells. Both ERCC1-XPF and MSH2-MSH3 bind to Z-DNA-forming sequences, though ERCC1-XPF recruitment to Z-DNA is dependent on MSH2-MSH3. Moreover, ERCC1-XPF−dependent DNA strand-breaks occur near the Z-DNA-forming region in human cell extracts, and we model these interactions at the sub-molecular level. We propose a relationship in which these complexes recognize and process Z-DNA in eukaryotes, representing a mechanism of Z-DNA-induced genomic instability.

Suggested Citation

  • Jennifer A. McKinney & Guliang Wang & Anirban Mukherjee & Laura Christensen & Sai H. Sankara Subramanian & Junhua Zhao & Karen M. Vasquez, 2020. "Distinct DNA repair pathways cause genomic instability at alternative DNA structures," Nature Communications, Nature, vol. 11(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-019-13878-9
    DOI: 10.1038/s41467-019-13878-9
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