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MAP3Kinase-dependent SnRK2-kinase activation is required for abscisic acid signal transduction and rapid osmotic stress response

Author

Listed:
  • Yohei Takahashi

    (University of California San Diego)

  • Jingbo Zhang

    (University of California San Diego
    China Agricultural University)

  • Po-Kai Hsu

    (University of California San Diego)

  • Paulo H. O. Ceciliato

    (University of California San Diego)

  • Li Zhang

    (University of California San Diego)

  • Guillaume Dubeaux

    (University of California San Diego)

  • Shintaro Munemasa

    (Okayama University, Tsushima-Naka)

  • Chennan Ge

    (University of California San Diego)

  • Yunde Zhao

    (University of California San Diego)

  • Felix Hauser

    (University of California San Diego)

  • Julian I. Schroeder

    (University of California San Diego)

Abstract

Abiotic stresses, including drought and salinity, trigger a complex osmotic-stress and abscisic acid (ABA) signal transduction network. The core ABA signalling components are snf1-related protein kinase2s (SnRK2s), which are activated by ABA-triggered inhibition of type-2C protein-phosphatases (PP2Cs). SnRK2 kinases are also activated by a rapid, largely unknown, ABA-independent osmotic-stress signalling pathway. Here, through a combination of a redundancy-circumventing genetic screen and biochemical analyses, we have identified functionally-redundant MAPKK-kinases (M3Ks) that are necessary for activation of SnRK2 kinases. These M3Ks phosphorylate a specific SnRK2/OST1 site, which is indispensable for ABA-induced reactivation of PP2C-dephosphorylated SnRK2 kinases. ABA-triggered SnRK2 activation, transcription factor phosphorylation and SLAC1 activation require these M3Ks in vitro and in plants. M3K triple knock-out plants show reduced ABA sensitivity and strongly impaired rapid osmotic-stress-induced SnRK2 activation. These findings demonstrate that this M3K clade is required for ABA- and osmotic-stress-activation of SnRK2 kinases, enabling robust ABA and osmotic stress signal transduction.

Suggested Citation

  • Yohei Takahashi & Jingbo Zhang & Po-Kai Hsu & Paulo H. O. Ceciliato & Li Zhang & Guillaume Dubeaux & Shintaro Munemasa & Chennan Ge & Yunde Zhao & Felix Hauser & Julian I. Schroeder, 2020. "MAP3Kinase-dependent SnRK2-kinase activation is required for abscisic acid signal transduction and rapid osmotic stress response," Nature Communications, Nature, vol. 11(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-019-13875-y
    DOI: 10.1038/s41467-019-13875-y
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    Cited by:

    1. Josep Vilarrasa-Blasi & Tamara Vellosillo & Robert E. Jinkerson & Friedrich Fauser & Tingting Xiang & Benjamin B. Minkoff & Lianyong Wang & Kiril Kniazev & Michael Guzman & Jacqueline Osaki & Gregory , 2024. "Multi-omics analysis of green lineage osmotic stress pathways unveils crucial roles of different cellular compartments," Nature Communications, Nature, vol. 15(1), pages 1-16, December.

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