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Quantitative live cell imaging reveals influenza virus manipulation of Rab11A transport through reduced dynein association

Author

Listed:
  • Amar R. Bhagwat

    (University of Pittsburgh School of Medicine)

  • Valerie Le Sage

    (University of Pittsburgh School of Medicine)

  • Eric Nturibi

    (University of Pittsburgh School of Medicine)

  • Katarzyna Kulej

    (The Children’s Hospital of Philadelphia Research Institute)

  • Jennifer Jones

    (University of Pittsburgh School of Medicine)

  • Min Guo

    (National Institutes of Health)

  • Eui Tae Kim

    (The Children’s Hospital of Philadelphia Research Institute)

  • Benjamin A. Garcia

    (University of Pennsylvania Perelman School of Medicine
    University of Pennsylvania Perelman School of Medicine)

  • Matthew D. Weitzman

    (The Children’s Hospital of Philadelphia Research Institute
    University of Pennsylvania Perelman School of Medicine
    University of Pennsylvania Perelman School of Medicine)

  • Hari Shroff

    (National Institutes of Health)

  • Seema S. Lakdawala

    (University of Pittsburgh School of Medicine
    University of Pittsburgh School of Medicine)

Abstract

Assembly of infectious influenza A viruses (IAV) is a complex process involving transport from the nucleus to the plasma membrane. Rab11A-containing recycling endosomes have been identified as a platform for intracellular transport of viral RNA (vRNA). Here, using high spatiotemporal resolution light-sheet microscopy (~1.4 volumes/second, 330 nm isotropic resolution), we quantify Rab11A and vRNA movement in live cells during IAV infection and report that IAV infection decreases speed and increases arrest of Rab11A. Unexpectedly, infection with respiratory syncytial virus alters Rab11A motion in a manner opposite to IAV, suggesting that Rab11A is a common host component that is differentially manipulated by respiratory RNA viruses. Using two-color imaging we demonstrate co-transport of Rab11A and IAV vRNA in infected cells and provide direct evidence that vRNA-associated Rab11A have altered transport. The mechanism of altered Rab11A movement is likely related to a decrease in dynein motors bound to Rab11A vesicles during IAV infection.

Suggested Citation

  • Amar R. Bhagwat & Valerie Le Sage & Eric Nturibi & Katarzyna Kulej & Jennifer Jones & Min Guo & Eui Tae Kim & Benjamin A. Garcia & Matthew D. Weitzman & Hari Shroff & Seema S. Lakdawala, 2020. "Quantitative live cell imaging reveals influenza virus manipulation of Rab11A transport through reduced dynein association," Nature Communications, Nature, vol. 11(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-019-13838-3
    DOI: 10.1038/s41467-019-13838-3
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    Cited by:

    1. Yuen-Yan Chang & Camila Valenzuela & Arthur Lensen & Noelia Lopez-Montero & Saima Sidik & John Salogiannis & Jost Enninga & John Rohde, 2024. "Microtubules provide force to promote membrane uncoating in vacuolar escape for a cyto-invasive bacterial pathogen," Nature Communications, Nature, vol. 15(1), pages 1-12, December.

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