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Structural basis for Fullerene geometry in a human endogenous retrovirus capsid

Author

Listed:
  • Oliver Acton

    (The Francis Crick Institute
    University of Washington)

  • Tim Grant

    (The Francis Crick Institute
    Howard Hughes Medical Institute)

  • Giuseppe Nicastro

    (The Francis Crick Institute)

  • Neil J. Ball

    (The Francis Crick Institute)

  • David C. Goldstone

    (The Francis Crick Institute
    University of Auckland)

  • Laura E. Robertson

    (The Francis Crick Institute)

  • Kasim Sader

    (The Francis Crick Institute
    Thermo Fisher Scientific Materials and Structural Analysis)

  • Andrea Nans

    (The Francis Crick Institute)

  • Andres Ramos

    (MRC National Institute for Medical Research
    University College London)

  • Jonathan P. Stoye

    (The Francis Crick Institute
    Imperial College London)

  • Ian A. Taylor

    (The Francis Crick Institute)

  • Peter B. Rosenthal

    (The Francis Crick Institute)

Abstract

The HML2 (HERV-K) group constitutes the most recently acquired family of human endogenous retroviruses, with many proviruses less than one million years old. Many maintain intact open reading frames and provirus expression together with HML2 particle formation are observed in early stage human embryo development and are associated with pluripotency as well as inflammatory disease, cancers and HIV-1 infection. Here, we reconstruct the core structural protein (CA) of an HML2 retrovirus, assemble particles in vitro and employ single particle cryogenic electron microscopy (cryo-EM) to determine structures of four classes of CA Fullerene shell assemblies. These icosahedral and capsular assemblies reveal at high-resolution the molecular interactions that allow CA to form both pentamers and hexamers and show how invariant pentamers and structurally plastic hexamers associate to form the unique polyhedral structures found in retroviral cores.

Suggested Citation

  • Oliver Acton & Tim Grant & Giuseppe Nicastro & Neil J. Ball & David C. Goldstone & Laura E. Robertson & Kasim Sader & Andrea Nans & Andres Ramos & Jonathan P. Stoye & Ian A. Taylor & Peter B. Rosentha, 2019. "Structural basis for Fullerene geometry in a human endogenous retrovirus capsid," Nature Communications, Nature, vol. 10(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-13786-y
    DOI: 10.1038/s41467-019-13786-y
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    Cited by:

    1. Anna-Sophia Krebs & Hsuan-Fu Liu & Ye Zhou & Juan S. Rey & Lev Levintov & Juan Shen & Andrew Howe & Juan R. Perilla & Alberto Bartesaghi & Peijun Zhang, 2023. "Molecular architecture and conservation of an immature human endogenous retrovirus," Nature Communications, Nature, vol. 14(1), pages 1-11, December.

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