IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v10y2019i1d10.1038_s41467-019-13598-0.html
   My bibliography  Save this article

MyoD induced enhancer RNA interacts with hnRNPL to activate target gene transcription during myogenic differentiation

Author

Listed:
  • Yu Zhao

    (The Chinese University of Hong Kong)

  • Jiajian Zhou

    (The Chinese University of Hong Kong
    Southern Medical University)

  • Liangqiang He

    (The Chinese University of Hong Kong)

  • Yuying Li

    (The Chinese University of Hong Kong)

  • Jie Yuan

    (The Chinese University of Hong Kong)

  • Kun Sun

    (The Chinese University of Hong Kong
    Shenzhen Bay Laboratory)

  • Xiaona Chen

    (The Chinese University of Hong Kong)

  • Xichen Bao

    (Chinese Academy of Sciences)

  • Miguel A. Esteban

    (Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences)

  • Hao Sun

    (The Chinese University of Hong Kong)

  • Huating Wang

    (The Chinese University of Hong Kong)

Abstract

Emerging evidence supports roles of enhancer RNAs (eRNAs) in regulating target gene. Here, we study eRNA regulation and function during skeletal myoblast differentiation. We provide a panoramic view of enhancer transcription and categorization of eRNAs. Master transcription factor MyoD is crucial in activating eRNA production. Super enhancer (se) generated seRNA-1 and -2 promote myogenic differentiation in vitro and in vivo. seRNA-1 regulates expression levels of two nearby genes, myoglobin (Mb) and apolipoprotein L6 (Apol6), by binding to heterogeneous nuclear ribonucleoprotein L (hnRNPL). A CAAA tract on seRNA-1 is essential in mediating seRNA-1/hnRNPL binding and function. Disruption of seRNA-1-hnRNPL interaction attenuates Pol II and H3K36me3 deposition at the Mb locus, in coincidence with the reduction of its transcription. Furthermore, analyses of hnRNPL binding transcriptome-wide reveal its association with eRNAs is a general phenomenon in multiple cells. Collectively, we propose that eRNA-hnRNPL interaction represents a mechanism contributing to target mRNA activation.

Suggested Citation

  • Yu Zhao & Jiajian Zhou & Liangqiang He & Yuying Li & Jie Yuan & Kun Sun & Xiaona Chen & Xichen Bao & Miguel A. Esteban & Hao Sun & Huating Wang, 2019. "MyoD induced enhancer RNA interacts with hnRNPL to activate target gene transcription during myogenic differentiation," Nature Communications, Nature, vol. 10(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-13598-0
    DOI: 10.1038/s41467-019-13598-0
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-019-13598-0
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-019-13598-0?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Yuwei Zhang & Jieyu Zhao & Xiaona Chen & Yulong Qiao & Jinjin Kang & Xiaofan Guo & Feng Yang & Kaixin Lyu & Yiliang Ding & Yu Zhao & Hao Sun & Chun-Kit Kwok & Huating Wang, 2024. "DHX36 binding induces RNA structurome remodeling and regulates RNA abundance via m6A reader YTHDF1," Nature Communications, Nature, vol. 15(1), pages 1-19, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-13598-0. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.