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Molecular insight into RNA polymerase I promoter recognition and promoter melting

Author

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  • Yashar Sadian

    (Structural and Computational Biology Unit
    University of Geneva)

  • Florence Baudin

    (Structural and Computational Biology Unit)

  • Lucas Tafur

    (Structural and Computational Biology Unit
    Faculty of Biosciences
    University of Geneva Sciences III)

  • Brice Murciano

    (Structural and Computational Biology Unit)

  • Rene Wetzel

    (Structural and Computational Biology Unit)

  • Felix Weis

    (Structural and Computational Biology Unit)

  • Christoph W. Müller

    (Structural and Computational Biology Unit)

Abstract

RNA polymerase I (Pol I) assembles with core factor (CF) and Rrn3 on the rDNA core promoter for transcription initiation. Here, we report cryo-EM structures of closed, intermediate and open Pol I initiation complexes from 2.7 to 3.7 Å resolution to visualize Pol I promoter melting and to structurally and biochemically characterize the recognition mechanism of Pol I promoter DNA. In the closed complex, double-stranded DNA runs outside the DNA-binding cleft. Rotation of CF and upstream DNA with respect to Pol I and Rrn3 results in the spontaneous loading and opening of the promoter followed by cleft closure and positioning of the Pol I A49 tandem winged helix domain (tWH) onto DNA. Conformational rearrangement of A49 tWH leads to a clash with Rrn3 to initiate complex disassembly and promoter escape. Comprehensive insight into the Pol I transcription initiation cycle allows comparisons with promoter opening by Pol II and Pol III.

Suggested Citation

  • Yashar Sadian & Florence Baudin & Lucas Tafur & Brice Murciano & Rene Wetzel & Felix Weis & Christoph W. Müller, 2019. "Molecular insight into RNA polymerase I promoter recognition and promoter melting," Nature Communications, Nature, vol. 10(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-13510-w
    DOI: 10.1038/s41467-019-13510-w
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