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Type I-F CRISPR-Cas resistance against virulent phages results in abortive infection and provides population-level immunity

Author

Listed:
  • Bridget N. J. Watson

    (University of Otago
    University of Exeter, Cornwall Campus)

  • Reuben B. Vercoe

    (University of Otago)

  • George P. C. Salmond

    (University of Cambridge)

  • Edze R. Westra

    (University of Exeter, Cornwall Campus)

  • Raymond H. J Staals

    (University of Otago
    Wageningen University and Research)

  • Peter C. Fineran

    (University of Otago
    University of Otago)

Abstract

Type I CRISPR-Cas systems are abundant and widespread adaptive immune systems in bacteria and can greatly enhance bacterial survival in the face of phage infection. Upon phage infection, some CRISPR-Cas immune responses result in bacterial dormancy or slowed growth, which suggests the outcomes for infected cells may vary between systems. Here we demonstrate that type I CRISPR immunity of Pectobacterium atrosepticum leads to suppression of two unrelated virulent phages, ɸTE and ɸM1. Immunity results in an abortive infection response, where infected cells do not survive, but viral propagation is severely decreased, resulting in population protection due to the reduced phage epidemic. Our findings challenge the view of CRISPR-Cas as a system that protects the individual cell and supports growing evidence of abortive infection by some types of CRISPR-Cas systems.

Suggested Citation

  • Bridget N. J. Watson & Reuben B. Vercoe & George P. C. Salmond & Edze R. Westra & Raymond H. J Staals & Peter C. Fineran, 2019. "Type I-F CRISPR-Cas resistance against virulent phages results in abortive infection and provides population-level immunity," Nature Communications, Nature, vol. 10(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-13445-2
    DOI: 10.1038/s41467-019-13445-2
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