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Permeability of membranes in the liquid ordered and liquid disordered phases

Author

Listed:
  • An Ghysels

    (Ghent University)

  • Andreas Krämer

    (National Institutes of Health)

  • Richard M. Venable

    (National Institutes of Health)

  • Walter E. Teague

    (National Institutes of Health)

  • Edward Lyman

    (University of Delaware)

  • Klaus Gawrisch

    (National Institutes of Health)

  • Richard W. Pastor

    (National Institutes of Health)

Abstract

The functional significance of ordered nanodomains (or rafts) in cholesterol rich eukaryotic cell membranes has only begun to be explored. This study exploits the correspondence of cellular rafts and liquid ordered (Lo) phases of three-component lipid bilayers to examine permeability. Molecular dynamics simulations of Lo phase dipalmitoylphosphatidylcholine (DPPC), dioleoylphosphatidylcholine (DOPC), and cholesterol show that oxygen and water transit a leaflet through the DOPC and cholesterol rich boundaries of hexagonally packed DPPC microdomains, freely diffuse along the bilayer midplane, and escape the membrane along the boundary regions. Electron paramagnetic resonance experiments provide critical validation: the measured ratio of oxygen concentrations near the midplanes of liquid disordered (Ld) and Lo bilayers of DPPC/DOPC/cholesterol is 1.75 ± 0.35, in very good agreement with 1.3 ± 0.3 obtained from simulation. The results show how cellular rafts can be structurally rigid signaling platforms while remaining nearly as permeable to small molecules as the Ld phase.

Suggested Citation

  • An Ghysels & Andreas Krämer & Richard M. Venable & Walter E. Teague & Edward Lyman & Klaus Gawrisch & Richard W. Pastor, 2019. "Permeability of membranes in the liquid ordered and liquid disordered phases," Nature Communications, Nature, vol. 10(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-13432-7
    DOI: 10.1038/s41467-019-13432-7
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    Cited by:

    1. Jacopo Frallicciardi & Josef Melcr & Pareskevi Siginou & Siewert J. Marrink & Bert Poolman, 2022. "Membrane thickness, lipid phase and sterol type are determining factors in the permeability of membranes to small solutes," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    2. Amy Rice & Sourav Haldar & Eric Wang & Paul S. Blank & Sergey A. Akimov & Timur R. Galimzyanov & Richard W. Pastor & Joshua Zimmerberg, 2022. "Planar aggregation of the influenza viral fusion peptide alters membrane structure and hydration, promoting poration," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    3. Dominik Ruppelt & Marius F. W. Trollmann & Taulant Dema & Sebastian N. Wirtz & Hendrik Flegel & Sophia Mönnikes & Stephanie Grond & Rainer A. Böckmann & Claudia Steinem, 2024. "The antimicrobial fibupeptide lugdunin forms water-filled channel structures in lipid membranes," Nature Communications, Nature, vol. 15(1), pages 1-16, December.

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