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Dynamic constriction and fission of endoplasmic reticulum membranes by reticulon

Author

Listed:
  • Javier Espadas

    (Biofisika Institute (CSIC, UPV/EHU) and Department of Biochemistry and Molecular, Biology, University of the Basque Country)

  • Diana Pendin

    (Scientific Institute, IRCCS E. Medea, Laboratory of Molecular Biology, Bosisio Parini
    Neuroscience Institute, Italian National Research Council (CNR))

  • Rebeca Bocanegra

    (IMDEA Nanociencia, C/Faraday 9, Ciudad Universitaria de Cantoblanco)

  • Artur Escalada

    (Biofisika Institute (CSIC, UPV/EHU) and Department of Biochemistry and Molecular, Biology, University of the Basque Country)

  • Giulia Misticoni

    (Scientific Institute, IRCCS E. Medea, Laboratory of Molecular Biology, Bosisio Parini)

  • Tatiana Trevisan

    (Scientific Institute, IRCCS E. Medea, Laboratory of Molecular Biology, Bosisio Parini)

  • Ariana Velasco del Olmo

    (Biofisika Institute (CSIC, UPV/EHU) and Department of Biochemistry and Molecular, Biology, University of the Basque Country)

  • Aldo Montagna

    (Scientific Institute, IRCCS E. Medea, Laboratory of Molecular Biology, Bosisio Parini)

  • Sergio Bova

    (Department of Pharmaceutical and Pharmacological Sciences, University of Padova)

  • Borja Ibarra

    (IMDEA Nanociencia, C/Faraday 9, Ciudad Universitaria de Cantoblanco
    Nanobiotecnología (IMDEA-Nanociencia) Unidad Asociada al Centro Nacional de Biotecnologia (CSIC))

  • Peter I. Kuzmin

    (A.N. Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences)

  • Pavel V. Bashkirov

    (Federal Research and Clinical Centre of Physical-Chemical Medicine)

  • Anna V. Shnyrova

    (Biofisika Institute (CSIC, UPV/EHU) and Department of Biochemistry and Molecular, Biology, University of the Basque Country)

  • Vadim A. Frolov

    (Biofisika Institute (CSIC, UPV/EHU) and Department of Biochemistry and Molecular, Biology, University of the Basque Country
    IKERBASQUE, Basque Foundation for Science)

  • Andrea Daga

    (Scientific Institute, IRCCS E. Medea, Laboratory of Molecular Biology, Bosisio Parini)

Abstract

The endoplasmic reticulum (ER) is a continuous cell-wide membrane network. Network formation has been associated with proteins producing membrane curvature and fusion, such as reticulons and atlastin. Regulated network fragmentation, occurring in different physiological contexts, is less understood. Here we find that the ER has an embedded fragmentation mechanism based upon the ability of reticulon to produce fission of elongating network branches. In Drosophila, Rtnl1-facilitated fission is counterbalanced by atlastin-driven fusion, with the prevalence of Rtnl1 leading to ER fragmentation. Ectopic expression of Drosophila reticulon in COS-7 cells reveals individual fission events in dynamic ER tubules. Consistently, in vitro analyses show that reticulon produces velocity-dependent constriction of lipid nanotubes leading to stochastic fission via a hemifission mechanism. Fission occurs at elongation rates and pulling force ranges intrinsic to the ER, thus suggesting a principle whereby the dynamic balance between fusion and fission controlling organelle morphology depends on membrane motility.

Suggested Citation

  • Javier Espadas & Diana Pendin & Rebeca Bocanegra & Artur Escalada & Giulia Misticoni & Tatiana Trevisan & Ariana Velasco del Olmo & Aldo Montagna & Sergio Bova & Borja Ibarra & Peter I. Kuzmin & Pavel, 2019. "Dynamic constriction and fission of endoplasmic reticulum membranes by reticulon," Nature Communications, Nature, vol. 10(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-13327-7
    DOI: 10.1038/s41467-019-13327-7
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