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SERS discrimination of single DNA bases in single oligonucleotides by electro-plasmonic trapping

Author

Listed:
  • Jian-An Huang

    (Istituto Italiano di Tecnologia)

  • Mansoureh Z. Mousavi

    (Istituto Italiano di Tecnologia)

  • Yingqi Zhao

    (Istituto Italiano di Tecnologia)

  • Aliaksandr Hubarevich

    (Istituto Italiano di Tecnologia)

  • Fatima Omeis

    (Istituto Italiano di Tecnologia)

  • Giorgia Giovannini

    (Istituto Italiano di Tecnologia)

  • Moritz Schütte

    (Alacris Theranostics GmbH)

  • Denis Garoli

    (Istituto Italiano di Tecnologia
    AB ANALITICA s.r.l.)

  • Francesco De Angelis

    (Istituto Italiano di Tecnologia)

Abstract

Surface-enhanced Raman spectroscopy (SERS) sensing of DNA bases by plasmonic nanopores could pave a way to novel methods for DNA analyses and new generation single-molecule sequencing platforms. The SERS discrimination of single DNA bases depends critically on the time that a DNA strand resides within the plasmonic hot spot. In fact, DNA molecules flow through the nanopores so rapidly that the SERS signals collected are not sufficient for single-molecule analysis. Here, we report an approach to control the residence time of molecules in the hot spot by an electro-plasmonic trapping effect. By directly adsorbing molecules onto a gold nanoparticle and then trapping the single nanoparticle in a plasmonic nanohole up to several minutes, we demonstrate single-molecule SERS detection of all four DNA bases as well as discrimination of single nucleobases in a single oligonucleotide. Our method can be extended easily to label-free sensing of single-molecule amino acids and proteins.

Suggested Citation

  • Jian-An Huang & Mansoureh Z. Mousavi & Yingqi Zhao & Aliaksandr Hubarevich & Fatima Omeis & Giorgia Giovannini & Moritz Schütte & Denis Garoli & Francesco De Angelis, 2019. "SERS discrimination of single DNA bases in single oligonucleotides by electro-plasmonic trapping," Nature Communications, Nature, vol. 10(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-13242-x
    DOI: 10.1038/s41467-019-13242-x
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    Cited by:

    1. Jianxin Yang & Tianle Pan & Zhenming Xie & Wu Yuan & Ho-Pui Ho, 2024. "In-tube micro-pyramidal silicon nanopore for inertial-kinetic sensing of single molecules," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    2. Wenhao Fu & Huanyu Chi & Xin Dai & Hongni Zhu & Vince St. Dollente Mesias & Wei Liu & Jinqing Huang, 2023. "Efficient optical plasmonic tweezer-controlled single-molecule SERS characterization of pH-dependent amylin species in aqueous milieus," Nature Communications, Nature, vol. 14(1), pages 1-13, December.

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