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Meiotic crossovers characterized by haplotype-specific chromosome painting in maize

Author

Listed:
  • Lívia Martins

    (Michigan State University
    Michigan State University)

  • Fan Yu

    (Michigan State University
    Michigan State University
    Fujian Agriculture and Forestry University)

  • Hainan Zhao

    (Michigan State University
    Michigan State University)

  • Tesia Dennison

    (Iowa State University)

  • Nick Lauter

    (Iowa State University
    Iowa State University)

  • Haiyan Wang

    (Michigan State University
    Michigan State University)

  • Zuhu Deng

    (Fujian Agriculture and Forestry University)

  • Addie Thompson

    (Michigan State University
    Michigan State University AgBioResearch)

  • Kassandra Semrau

    (Arbor Biosciences
    University of Michigan-Dearborn)

  • Jean-Marie Rouillard

    (Arbor Biosciences
    University of Michigan)

  • James A. Birchler

    (University of Missouri)

  • Jiming Jiang

    (Michigan State University
    Michigan State University
    Michigan State University AgBioResearch)

Abstract

Meiotic crossovers (COs) play a critical role in generating genetic variation and maintaining faithful segregation of homologous chromosomes during meiosis. We develop a haplotype-specific fluorescence in situ hybridization (FISH) technique that allows visualization of COs directly on metaphase chromosomes. Oligonucleotides (oligos) specific to chromosome 10 of maize inbreds B73 and Mo17, respectively, are synthesized and labeled as FISH probes. The parental and recombinant chromosome 10 in B73 x Mo17 F1 hybrids and F2 progenies can be unambiguously identified by haplotype-specific FISH. Analysis of 58 F2 plants reveals lack of COs in the entire proximal half of chromosome 10. However, we detect COs located in regions very close to the centromere in recombinant inbred lines from an intermated B73 x Mo17 population, suggesting effective accumulation of COs in recombination-suppressed chromosomal regions through intermating and the potential to generate favorable allelic combinations of genes residing in these regions.

Suggested Citation

  • Lívia Martins & Fan Yu & Hainan Zhao & Tesia Dennison & Nick Lauter & Haiyan Wang & Zuhu Deng & Addie Thompson & Kassandra Semrau & Jean-Marie Rouillard & James A. Birchler & Jiming Jiang, 2019. "Meiotic crossovers characterized by haplotype-specific chromosome painting in maize," Nature Communications, Nature, vol. 10(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-12646-z
    DOI: 10.1038/s41467-019-12646-z
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