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Synthetic protein-conductive membrane nanopores built with DNA

Author

Listed:
  • Tim Diederichs

    (Goethe University Frankfurt)

  • Genevieve Pugh

    (University College London)

  • Adam Dorey

    (University College London)

  • Yongzheng Xing

    (University College London)

  • Jonathan R. Burns

    (University College London)

  • Quoc Hung Nguyen

    (Technical University of Munich)

  • Marc Tornow

    (Technical University of Munich
    Fraunhofer Research Institution for Microsystems and Solid State Technologies (EMFT)
    Ludwig-Maximilians-University)

  • Robert Tampé

    (Goethe University Frankfurt)

  • Stefan Howorka

    (University College London)

Abstract

Nanopores are key in portable sequencing and research given their ability to transport elongated DNA or small bioactive molecules through narrow transmembrane channels. Transport of folded proteins could lead to similar scientific and technological benefits. Yet this has not been realised due to the shortage of wide and structurally defined natural pores. Here we report that a synthetic nanopore designed via DNA nanotechnology can accommodate folded proteins. Transport of fluorescent proteins through single pores is kinetically analysed using massively parallel optical readout with transparent silicon-on-insulator cavity chips vs. electrical recordings to reveal an at least 20-fold higher speed for the electrically driven movement. Pores nevertheless allow a high diffusive flux of more than 66 molecules per second that can also be directed beyond equillibria. The pores may be exploited to sense diagnostically relevant proteins with portable analysis technology, to create molecular gates for drug delivery, or to build synthetic cells.

Suggested Citation

  • Tim Diederichs & Genevieve Pugh & Adam Dorey & Yongzheng Xing & Jonathan R. Burns & Quoc Hung Nguyen & Marc Tornow & Robert Tampé & Stefan Howorka, 2019. "Synthetic protein-conductive membrane nanopores built with DNA," Nature Communications, Nature, vol. 10(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-12639-y
    DOI: 10.1038/s41467-019-12639-y
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    Cited by:

    1. Smrithi Krishnan R & Kalyanashis Jana & Amina H. Shaji & Karthika S. Nair & Anjali Devi Das & Devika Vikraman & Harsha Bajaj & Ulrich Kleinekathöfer & Kozhinjampara R. Mahendran, 2022. "Assembly of transmembrane pores from mirror-image peptides," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    2. Katya Ahmad & Abid Javed & Conor Lanphere & Peter V. Coveney & Elena V. Orlova & Stefan Howorka, 2023. "Structure and dynamics of an archetypal DNA nanoarchitecture revealed via cryo-EM and molecular dynamics simulations," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    3. Swarup Dey & Adam Dorey & Leeza Abraham & Yongzheng Xing & Irene Zhang & Fei Zhang & Stefan Howorka & Hao Yan, 2022. "A reversibly gated protein-transporting membrane channel made of DNA," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    4. Qi Yang & Xu Chang & Jung Yeon Lee & Minu Saji & Fei Zhang, 2023. "DNA T-shaped crossover tiles for 2D tessellation and nanoring reconfiguration," Nature Communications, Nature, vol. 14(1), pages 1-11, December.

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