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Photoactivation of silicon rhodamines via a light-induced protonation

Author

Listed:
  • Michelle S. Frei

    (Max Planck Institute for Medical Research
    École Polytechnique Fédérale de Lausanne (EPFL))

  • Philipp Hoess

    (European Molecular Biology Laboratory (EMBL)
    Collaboration for joint PhD degree between EMBL and Heidelberg University, Faculty of Biosciences)

  • Marko Lampe

    (European Molecular Biology Laboratory (EMBL))

  • Bianca Nijmeijer

    (European Molecular Biology Laboratory (EMBL))

  • Moritz Kueblbeck

    (European Molecular Biology Laboratory (EMBL))

  • Jan Ellenberg

    (European Molecular Biology Laboratory (EMBL))

  • Hubert Wadepohl

    (University of Heidelberg)

  • Jonas Ries

    (European Molecular Biology Laboratory (EMBL))

  • Stefan Pitsch

    (Spirochrome AG)

  • Luc Reymond

    (École Polytechnique Fédérale de Lausanne (EPFL)
    National Centre of Competence in Research (NCCR) in Chemical Biology)

  • Kai Johnsson

    (Max Planck Institute for Medical Research
    École Polytechnique Fédérale de Lausanne (EPFL)
    National Centre of Competence in Research (NCCR) in Chemical Biology)

Abstract

Photoactivatable fluorophores are important for single-particle tracking and super-resolution microscopy. Here we present a photoactivatable fluorophore that forms a bright silicon rhodamine derivative through a light-dependent protonation. In contrast to other photoactivatable fluorophores, no caging groups are required, nor are there any undesired side-products released. Using this photoactivatable fluorophore, we create probes for HaloTag and actin for live-cell single-molecule localization microscopy and single-particle tracking experiments. The unusual mechanism of photoactivation and the fluorophore’s outstanding spectroscopic properties make it a powerful tool for live-cell super-resolution microscopy.

Suggested Citation

  • Michelle S. Frei & Philipp Hoess & Marko Lampe & Bianca Nijmeijer & Moritz Kueblbeck & Jan Ellenberg & Hubert Wadepohl & Jonas Ries & Stefan Pitsch & Luc Reymond & Kai Johnsson, 2019. "Photoactivation of silicon rhodamines via a light-induced protonation," Nature Communications, Nature, vol. 10(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-12480-3
    DOI: 10.1038/s41467-019-12480-3
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    Cited by:

    1. Jan-Erik Bredfeldt & Joanna Oracz & Kamila A. Kiszka & Thea Moosmayer & Michael Weber & Steffen J. Sahl & Stefan W. Hell, 2024. "Bleaching protection and axial sectioning in fluorescence nanoscopy through two-photon activation at 515 nm," Nature Communications, Nature, vol. 15(1), pages 1-13, December.

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