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Monitoring cytosolic H2O2 fluctuations arising from altered plasma membrane gradients or from mitochondrial activity

Author

Listed:
  • Mercè Carmona

    (Universitat Pompeu Fabra)

  • Laura de Cubas

    (Universitat Pompeu Fabra)

  • Eric Bautista

    (Universitat Pompeu Fabra)

  • Marta Moral-Blanch

    (Universitat Pompeu Fabra)

  • Iria Medraño-Fernández

    (Università Vita-Salute San Raffaele)

  • Roberto Sitia

    (Università Vita-Salute San Raffaele)

  • Susanna Boronat

    (Universitat Pompeu Fabra)

  • José Ayté

    (Universitat Pompeu Fabra)

  • Elena Hidalgo

    (Universitat Pompeu Fabra)

Abstract

Genetically encoded probes monitoring H2O2 fluctuations in living organisms are key to decipher redox signaling events. Here we use a new probe, roGFP2-Tpx1.C169S, to monitor pre-toxic fluctuations of peroxides in fission yeast, where the concentrations linked to signaling or to toxicity have been established. This probe is able to detect nanomolar fluctuations of intracellular H2O2 caused by extracellular peroxides; expression of human aquaporin 8 channels H2O2 entry into fission yeast decreasing membrane gradients. The probe also detects H2O2 bursts from mitochondria after addition of electron transport chain inhibitors, the extent of probe oxidation being proportional to the mitochondrial activity. The oxidation of this probe is an indicator of steady-state levels of H2O2 in different genetic backgrounds. Metabolic reprogramming during growth in low-glucose media causes probe reduction due to the activation of antioxidant cascades. We demonstrate how peroxiredoxin-based probes can be used to monitor physiological H2O2 fluctuations.

Suggested Citation

  • Mercè Carmona & Laura de Cubas & Eric Bautista & Marta Moral-Blanch & Iria Medraño-Fernández & Roberto Sitia & Susanna Boronat & José Ayté & Elena Hidalgo, 2019. "Monitoring cytosolic H2O2 fluctuations arising from altered plasma membrane gradients or from mitochondrial activity," Nature Communications, Nature, vol. 10(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-12475-0
    DOI: 10.1038/s41467-019-12475-0
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    Cited by:

    1. Daan M. K. Soest & Paulien E. Polderman & Wytze T. F. Toom & Janneke P. Keijer & Markus J. Roosmalen & Tim M. F. Leyten & Johannes Lehmann & Susan Zwakenberg & Sasha Henau & Ruben Boxtel & Boudewijn M, 2024. "Mitochondrial H2O2 release does not directly cause damage to chromosomal DNA," Nature Communications, Nature, vol. 15(1), pages 1-16, December.

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