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CENP-A nucleosome clusters form rosette-like structures around HJURP during G1

Author

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  • Leonid Andronov

    (Université de Strasbourg
    Institute of Genetics and of Molecular and Cellular Biology (IGBMC)
    Centre National de la Recherche Scientifique (CNRS)
    Institut National de la Santé et de la Recherche Médicale (Inserm))

  • Khalid Ouararhni

    (Institute of Genetics and of Molecular and Cellular Biology (IGBMC)
    Centre National de la Recherche Scientifique (CNRS)
    Institut National de la Santé et de la Recherche Médicale (Inserm)
    Université de Strasbourg)

  • Isabelle Stoll

    (Institute of Genetics and of Molecular and Cellular Biology (IGBMC)
    Centre National de la Recherche Scientifique (CNRS)
    Institut National de la Santé et de la Recherche Médicale (Inserm)
    Université de Strasbourg)

  • Bruno P. Klaholz

    (Université de Strasbourg
    Institute of Genetics and of Molecular and Cellular Biology (IGBMC)
    Centre National de la Recherche Scientifique (CNRS)
    Institut National de la Santé et de la Recherche Médicale (Inserm))

  • Ali Hamiche

    (Institute of Genetics and of Molecular and Cellular Biology (IGBMC)
    Centre National de la Recherche Scientifique (CNRS)
    Institut National de la Santé et de la Recherche Médicale (Inserm)
    Université de Strasbourg)

Abstract

CENP-A is an essential histone H3 variant that epigenetically marks the centromeric region of chromosomes. Here we show that CENP-A nucleosomes form characteristic clusters during the G1 phase of the cell cycle. 2D and 3D super-resolution microscopy and segmentation analysis reveal that these clusters encompass a globular rosette-like structure, which evolves into a more compact structure in late G1. The rosette-like clusters contain numerous CENP-A molecules and form a large cellular structure of ∼250–300 nm diameter with remarkably similar shapes for each centromere. Co-localization analysis shows that HJURP, the CENP-A chaperone, is located in the center of the rosette and serves as a nucleation point. The discovery of an HJURP-mediated CENP-A nucleation in human cells and its structural description provide important insights into the mechanism of CENP-A deposition and the organization of CENP-A chromatin in the centromeric region.

Suggested Citation

  • Leonid Andronov & Khalid Ouararhni & Isabelle Stoll & Bruno P. Klaholz & Ali Hamiche, 2019. "CENP-A nucleosome clusters form rosette-like structures around HJURP during G1," Nature Communications, Nature, vol. 10(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-12383-3
    DOI: 10.1038/s41467-019-12383-3
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    Cited by:

    1. Leonid Andronov & Mengting Han & Yanyu Zhu & Ashwin Balaji & Anish R. Roy & Andrew E. S. Barentine & Puja Patel & Jaishree Garhyan & Lei S. Qi & W. E. Moerner, 2024. "Nanoscale cellular organization of viral RNA and proteins in SARS-CoV-2 replication organelles," Nature Communications, Nature, vol. 15(1), pages 1-17, December.

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