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Flotillins promote T cell receptor sorting through a fast Rab5–Rab11 endocytic recycling axis

Author

Listed:
  • Gregory M. I. Redpath

    (University of New South Wales)

  • Manuela Ecker

    (University of New South Wales)

  • Natasha Kapoor-Kaushik

    (University of New South Wales)

  • Haig Vartoukian

    (University of New South Wales)

  • Michael Carnell

    (University of New South Wales)

  • Daryan Kempe

    (University of New South Wales)

  • Maté Biro

    (University of New South Wales)

  • Nicholas Ariotti

    (University of New South Wales
    University of New South Wales)

  • Jérémie Rossy

    (University of New South Wales
    Biotechnology Institute Thurgau at the University of Konstanz)

Abstract

The targeted endocytic recycling of the T cell receptor (TCR) to the immunological synapse is essential for T cell activation. Despite this, the mechanisms that underlie the sorting of internalised receptors into recycling endosomes remain poorly understood. To build a comprehensive picture of TCR recycling during T cell activation, we developed a suite of new imaging and quantification tools centred on photoactivation of fluorescent proteins. We show that the membrane-organising proteins, flotillin-1 and -2, are required for TCR to reach Rab5-positive endosomes immediately after endocytosis and for transfer from Rab5- to Rab11a-positive compartments. We further observe that after sorting into in Rab11a-positive vesicles, TCR recycles to the plasma membrane independent of flotillin expression. Our data suggest a mechanism whereby flotillins delineate a fast Rab5-Rab11a endocytic recycling axis and functionally contribute to regulate the spatial organisation of these endosomes.

Suggested Citation

  • Gregory M. I. Redpath & Manuela Ecker & Natasha Kapoor-Kaushik & Haig Vartoukian & Michael Carnell & Daryan Kempe & Maté Biro & Nicholas Ariotti & Jérémie Rossy, 2019. "Flotillins promote T cell receptor sorting through a fast Rab5–Rab11 endocytic recycling axis," Nature Communications, Nature, vol. 10(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-12352-w
    DOI: 10.1038/s41467-019-12352-w
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