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Linked-read sequencing of gametes allows efficient genome-wide analysis of meiotic recombination

Author

Listed:
  • Hequan Sun

    (Max Planck Institute for Plant Breeding Research)

  • Beth A. Rowan

    (University of California, Davis)

  • Pádraic J. Flood

    (Max Planck Institute for Plant Breeding Research)

  • Ronny Brandt

    (Max Planck Institute for Plant Breeding Research)

  • Janina Fuss

    (Max Planck Institute for Plant Breeding Research)

  • Angela M. Hancock

    (Max Planck Institute for Plant Breeding Research)

  • Richard W. Michelmore

    (University of California, Davis)

  • Bruno Huettel

    (Max Planck Institute for Plant Breeding Research)

  • Korbinian Schneeberger

    (Max Planck Institute for Plant Breeding Research)

Abstract

Meiotic crossovers (COs) ensure proper chromosome segregation and redistribute the genetic variation that is transmitted to the next generation. Large populations and the demand for genome-wide, fine-scale resolution challenge existing methods for CO identification. Taking advantage of linked-read sequencing, we develop a highly efficient method for genome-wide identification of COs at kilobase resolution in pooled recombinants. We first test this method using a pool of Arabidopsis F2 recombinants, and recapitulate results obtained from the same plants using individual whole-genome sequencing. By applying this method to a pool of pollen DNA from an F1 plant, we establish a highly accurate CO landscape without generating or sequencing a single recombinant plant. The simplicity of this approach enables the simultaneous generation and analysis of multiple CO landscapes, accelerating the pace at which mechanisms for the regulation of recombination can be elucidated through efficient comparisons of genotypic and environmental effects on recombination.

Suggested Citation

  • Hequan Sun & Beth A. Rowan & Pádraic J. Flood & Ronny Brandt & Janina Fuss & Angela M. Hancock & Richard W. Michelmore & Bruno Huettel & Korbinian Schneeberger, 2019. "Linked-read sequencing of gametes allows efficient genome-wide analysis of meiotic recombination," Nature Communications, Nature, vol. 10(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-12209-2
    DOI: 10.1038/s41467-019-12209-2
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