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A highly dynamic F-actin network regulates transport and recycling of micronemes in Toxoplasma gondii vacuoles

Author

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  • Javier Periz

    (University of Glasgow)

  • Mario Rosario

    (University of Glasgow)

  • Alexandra McStea

    (Research Complex at Harwell Science & Technology Facilities Council)

  • Simon Gras

    (Ludwig-Maximilians-University Munich)

  • Colin Loney

    (MRC-University of Glasgow Centre for Virus Research, Sir Michael Stoker Building)

  • Lin Wang

    (Research Complex at Harwell Science & Technology Facilities Council)

  • Marisa L. Martin-Fernandez

    (Research Complex at Harwell Science & Technology Facilities Council)

  • Markus Meissner

    (University of Glasgow
    Ludwig-Maximilians-University Munich)

Abstract

The obligate intracellular parasite Toxoplasma gondii replicates in an unusual process, described as internal budding. Multiple dausghter parasites are formed sequentially within a single mother cell, requiring replication and distribution of essential organelles such as micronemes. These organelles are thought to be formed de novo in the developing daughter cells. Using dual labelling of a microneme protein MIC2 and super-resolution microscopy, we show that micronemes are recycled from the mother to the forming daughter parasites using a highly dynamic F-actin network. While this recycling pathway is F-actin dependent, de novo synthesis of micronemes appears to be F-actin independent. The F-actin network connects individual parasites, supports long, multidirectional vesicular transport, and regulates transport, density and localisation of micronemal vesicles. The residual body acts as a storage and sorting station for these organelles. Our data describe an F-actin dependent mechanism in apicomplexans for transport and recycling of maternal organelles during intracellular development.

Suggested Citation

  • Javier Periz & Mario Rosario & Alexandra McStea & Simon Gras & Colin Loney & Lin Wang & Marisa L. Martin-Fernandez & Markus Meissner, 2019. "A highly dynamic F-actin network regulates transport and recycling of micronemes in Toxoplasma gondii vacuoles," Nature Communications, Nature, vol. 10(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-12136-2
    DOI: 10.1038/s41467-019-12136-2
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    Cited by:

    1. Kelli L. Hvorecny & Thomas E. Sladewski & Enrique M. Cruz & Justin M. Kollman & Aoife T. Heaslip, 2024. "Toxoplasma gondii actin filaments are tuned for rapid disassembly and turnover," Nature Communications, Nature, vol. 15(1), pages 1-16, December.
    2. Long Gui & William J. O’Shaughnessy & Kai Cai & Evan Reetz & Michael L. Reese & Daniela Nicastro, 2023. "Cryo-tomography reveals rigid-body motion and organization of apicomplexan invasion machinery," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    3. Ludek Koreny & Brandon N. Mercado-Saavedra & Christen M. Klinger & Konstantin Barylyuk & Simon Butterworth & Jennifer Hirst & Yolanda Rivera-Cuevas & Nathan R. Zaccai & Victoria J. C. Holzer & Andreas, 2023. "Stable endocytic structures navigate the complex pellicle of apicomplexan parasites," Nature Communications, Nature, vol. 14(1), pages 1-19, December.

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