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Continuous artificial synthesis of glucose precursor using enzyme-immobilized microfluidic reactors

Author

Listed:
  • Yujiao Zhu

    (The Hong Kong Polytechnic University
    University of Macau)

  • Ziyu Huang

    (Qilu University of Technology (Shandong Academy of Sciences))

  • Qingming Chen

    (The Hong Kong Polytechnic University)

  • Qian Wu

    (The Hong Kong Polytechnic University
    Shenzhen Research Institute of the Hong Kong Polytechnic University)

  • Xiaowen Huang

    (Qilu University of Technology (Shandong Academy of Sciences))

  • Pui-Kin So

    (The Hong Kong Polytechnic University)

  • Liyang Shao

    (Southern University of Science and Technology)

  • Zhongping Yao

    (The Hong Kong Polytechnic University
    Shenzhen Research Institute of the Hong Kong Polytechnic University)

  • Yanwei Jia

    (University of Macau
    University of Macau
    University of Macau)

  • Zhaohui Li

    (Sun Yat-Sen University
    Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai))

  • Weixing Yu

    (Chinese Academy of Sciences)

  • Yi Yang

    (Wuhan University)

  • Aoqun Jian

    (Taiyuan University of Technology)

  • Shengbo Sang

    (Taiyuan University of Technology)

  • Wendong Zhang

    (Taiyuan University of Technology)

  • Xuming Zhang

    (The Hong Kong Polytechnic University)

Abstract

Food production in green crops is severely limited by low activity and poor specificity of D-ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) in natural photosynthesis (NPS). This work presents a scientific solution to overcome this problem by immobilizing RuBisCO into a microfluidic reactor, which demonstrates a continuous production of glucose precursor at 13.8 μmol g−1 RuBisCO min−1 from CO2 and ribulose-1,5-bisphosphate. Experiments show that the RuBisCO immobilization significantly enhances enzyme stabilities (7.2 folds in storage stability, 6.7 folds in thermal stability), and also improves the reusability (90.4% activity retained after 5 cycles of reuse and 78.5% after 10 cycles). This work mimics the NPS pathway with scalable microreactors for continuous synthesis of glucose precursor using very small amount of RuBisCO. Although still far from industrial production, this work demonstrates artificial synthesis of basic food materials by replicating the light-independent reactions of NPS, which may hold the key to food crisis relief and future space colonization.

Suggested Citation

  • Yujiao Zhu & Ziyu Huang & Qingming Chen & Qian Wu & Xiaowen Huang & Pui-Kin So & Liyang Shao & Zhongping Yao & Yanwei Jia & Zhaohui Li & Weixing Yu & Yi Yang & Aoqun Jian & Shengbo Sang & Wendong Zhan, 2019. "Continuous artificial synthesis of glucose precursor using enzyme-immobilized microfluidic reactors," Nature Communications, Nature, vol. 10(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-12089-6
    DOI: 10.1038/s41467-019-12089-6
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    Cited by:

    1. Shanshan Zhang & Jiahui Sun & Dandan Feng & Huili Sun & Jinyu Cui & Xuexia Zeng & Yannan Wu & Guodong Luan & Xuefeng Lu, 2023. "Unlocking the potentials of cyanobacterial photosynthesis for directly converting carbon dioxide into glucose," Nature Communications, Nature, vol. 14(1), pages 1-14, December.

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