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A second open reading frame in human enterovirus determines viral replication in intestinal epithelial cells

Author

Listed:
  • Haoran Guo

    (Jilin University
    Jilin University)

  • Yan Li

    (Jilin University)

  • Guanchen Liu

    (Jilin University)

  • Yunhe Jiang

    (Jilin University)

  • Siyu Shen

    (Jilin University)

  • Ran Bi

    (Jilin University)

  • Honglan Huang

    (Jilin University)

  • Tong Cheng

    (Xiamen University)

  • Chunxi Wang

    (Jilin University)

  • Wei Wei

    (Jilin University
    Jilin University)

Abstract

Human enteroviruses (HEVs) of the family Picornaviridae, which comprises non-enveloped RNA viruses, are ubiquitous worldwide. The majority of EV proteins are derived from viral polyproteins encoded by a single open reading frame (ORF). Here, we characterize a second ORF in HEVs that is crucial for viral intestinal infection. Disruption of ORF2p expression decreases the replication capacity of EV-A71 in human intestinal epithelial cells (IECs). Ectopic expression of ORF2p proteins derived from diverse enteric enteroviruses sensitizes intestinal cells to the replication of ORF2p-defective EV-A71 and respiratory enterovirus EV-D68. We show that the highly conserved WIGHPV domain of ORF2p is important for ORF2p-dependent viral intestinal infection. ORF2p expression is required for EV-A71 particle release from IECs and can support productive EV-D68 infection in IECs by facilitating virus release. Our results indicate that ORF2p is a determining factor for enteric enterovirus replication in IECs.

Suggested Citation

  • Haoran Guo & Yan Li & Guanchen Liu & Yunhe Jiang & Siyu Shen & Ran Bi & Honglan Huang & Tong Cheng & Chunxi Wang & Wei Wei, 2019. "A second open reading frame in human enterovirus determines viral replication in intestinal epithelial cells," Nature Communications, Nature, vol. 10(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-12040-9
    DOI: 10.1038/s41467-019-12040-9
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    Cited by:

    1. Zecheng Zhong & Xiaosong Su & Kunyu Yang & Weida Huang & Jin Wang & Zhihao Zhuo & Jiyu Xiang & Lesi Lin & Shuizhen He & Tingdong Li & Jun Zhang & Shengxiang Ge & Shiyin Zhang & Ningshao Xia, 2024. "Sequence-specific nanoparticle barcode strategy for multiplex human enterovirus typing," Nature Communications, Nature, vol. 15(1), pages 1-9, December.

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