IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v10y2019i1d10.1038_s41467-019-12025-8.html
   My bibliography  Save this article

Polyunsaturated fatty acid production by Yarrowia lipolytica employing designed myxobacterial PUFA synthases

Author

Listed:
  • Katja Gemperlein

    (Helmholtz Institute for Pharmaceutical Research Saarland and Helmholtz Centre for Infection Research)

  • Demian Dietrich

    (Saarland University)

  • Michael Kohlstedt

    (Saarland University)

  • Gregor Zipf

    (ATG:biosynthetics GmbH)

  • Hubert S. Bernauer

    (ATG:biosynthetics GmbH)

  • Christoph Wittmann

    (Saarland University)

  • Silke C. Wenzel

    (Saarland University)

  • Rolf Müller

    (Helmholtz Institute for Pharmaceutical Research Saarland and Helmholtz Centre for Infection Research
    Saarland University)

Abstract

Long-chain polyunsaturated fatty acids (LC-PUFAs), particularly the omega-3 LC-PUFAs eicosapentaenoic acid (EPA), docosapentaenoic acid (DPA), and docosahexaenoic acid (DHA), have been associated with beneficial health effects. Consequently, sustainable sources have to be developed to meet the increasing demand for these PUFAs. Here, we demonstrate the design and construction of artificial PUFA biosynthetic gene clusters (BGCs) encoding polyketide synthase-like PUFA synthases from myxobacteria adapted for the oleaginous yeast Yarrowia lipolytica. Genomic integration and heterologous expression of unmodified or hybrid PUFA BGCs yielded different yeast strains with specific LC-PUFA production profiles at promising yield and thus valuable for the biotechnological production of distinct PUFAs. Nutrient screening revealed a strong enhancement of PUFA production, when cells were phosphate limited. This represents, to the best of our knowledge, highest concentration of DHA (16.8 %) in total fatty acids among all published PUFA-producing Y. lipolytica strains.

Suggested Citation

  • Katja Gemperlein & Demian Dietrich & Michael Kohlstedt & Gregor Zipf & Hubert S. Bernauer & Christoph Wittmann & Silke C. Wenzel & Rolf Müller, 2019. "Polyunsaturated fatty acid production by Yarrowia lipolytica employing designed myxobacterial PUFA synthases," Nature Communications, Nature, vol. 10(1), pages 1-12, December.
  • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-12025-8
    DOI: 10.1038/s41467-019-12025-8
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-019-12025-8
    File Function: Abstract
    Download Restriction: no

    File URL: https://libkey.io/10.1038/s41467-019-12025-8?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Dar, Rouf Ahmad & Tsui, To-Hung & Zhang, Le & Tong, Yen Wah & Sharon, Sigal & Shoseyov, Oded & Liu, Ronghou, 2024. "Fermentation of organic wastes through oleaginous microorganisms for lipid production - Challenges and opportunities," Renewable and Sustainable Energy Reviews, Elsevier, vol. 195(C).
    2. Magdalena Wróbel-Kwiatkowska & Waldemar Turski & Piotr Juszczyk & Agnieszka Kita & Waldemar Rymowicz, 2020. "Improved Production of Kynurenic Acid by Yarrowia lipolytica in Media Containing Different Honeys," Sustainability, MDPI, vol. 12(22), pages 1-9, November.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-12025-8. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.