IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v10y2019i1d10.1038_s41467-019-11999-9.html
   My bibliography  Save this article

Translational coupling via termination-reinitiation in archaea and bacteria

Author

Listed:
  • Madeleine Huber

    (Goethe University, Institute for Molecular Biosciences)

  • Guilhem Faure

    (National Institutes of Health
    Broad Institute of MIT and Harvard)

  • Sebastian Laass

    (Goethe University, Institute for Molecular Biosciences)

  • Esther Kolbe

    (Goethe University, Institute for Molecular Biosciences)

  • Kristina Seitz

    (Goethe University, Institute for Molecular Biosciences)

  • Christina Wehrheim

    (Goethe University, Institute for Molecular Biosciences)

  • Yuri I. Wolf

    (National Institutes of Health)

  • Eugene V. Koonin

    (National Institutes of Health)

  • Jörg Soppa

    (Goethe University, Institute for Molecular Biosciences)

Abstract

The genomes of many prokaryotes contain substantial fractions of gene pairs with overlapping stop and start codons (ATGA or TGATG). A potential benefit of overlapping gene pairs is translational coupling. In 720 genomes of archaea and bacteria representing all major phyla, we identify substantial, albeit highly variable, fractions of co-directed overlapping gene pairs. Various patterns are observed for the utilization of the SD motif for de novo initiation at upstream genes versus reinitiation at overlapping gene pairs. We experimentally test the predicted coupling in 9 gene pairs from the archaeon Haloferax volcanii and 5 gene pairs from the bacterium Escherichia coli. In 13 of 14 cases, translation of both genes is strictly coupled. Mutational analysis of SD motifs located upstream of the downstream genes indicate that the contribution of the SD to translational coupling widely varies from gene to gene. The nearly universal, abundant occurrence of overlapping gene pairs suggests that tight translational coupling is widespread in archaea and bacteria.

Suggested Citation

  • Madeleine Huber & Guilhem Faure & Sebastian Laass & Esther Kolbe & Kristina Seitz & Christina Wehrheim & Yuri I. Wolf & Eugene V. Koonin & Jörg Soppa, 2019. "Translational coupling via termination-reinitiation in archaea and bacteria," Nature Communications, Nature, vol. 10(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-11999-9
    DOI: 10.1038/s41467-019-11999-9
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-019-11999-9
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-019-11999-9?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Mikhail Metelev & Erik Lundin & Ivan L. Volkov & Arvid H. Gynnå & Johan Elf & Magnus Johansson, 2022. "Direct measurements of mRNA translation kinetics in living cells," Nature Communications, Nature, vol. 13(1), pages 1-13, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-11999-9. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.