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Non-classical tissue monocytes and two functionally distinct populations of interstitial macrophages populate the mouse lung

Author

Listed:
  • Joey Schyns

    (Liège University
    Liège University
    Liège University)

  • Qiang Bai

    (Liège University
    Liège University)

  • Cecilia Ruscitti

    (Liège University
    Liège University)

  • Coraline Radermecker

    (Liège University
    Liège University)

  • Sebastiaan Schepper

    (KU Leuven)

  • Svetoslav Chakarov

    (A*STAR, Biomedical Grove 8a)

  • Frédéric Farnir

    (Liège University
    Liège University)

  • Dimitri Pirottin

    (Liège University
    Liège University)

  • Florent Ginhoux

    (A*STAR, Biomedical Grove 8a
    Shanghai JiaoTong University School of Medicine)

  • Guy Boeckxstaens

    (KU Leuven)

  • Fabrice Bureau

    (Liège University
    Liège University
    WELBIO, Walloon Excellence in Life Sciences and Biotechnology)

  • Thomas Marichal

    (Liège University
    Liège University
    Liège University
    WELBIO, Walloon Excellence in Life Sciences and Biotechnology)

Abstract

Resident tissue macrophages (RTM) can fulfill various tasks during development, homeostasis, inflammation and repair. In the lung, non-alveolar RTM, called interstitial macrophages (IM), importantly contribute to tissue homeostasis but remain little characterized. Here we show, using single-cell RNA-sequencing (scRNA-seq), two phenotypically distinct subpopulations of long-lived monocyte-derived IM, i.e. CD206+ and CD206−IM, as well as a discrete population of extravasating CD64+CD16.2+ monocytes. CD206+ IM are peribronchial self-maintaining RTM that constitutively produce high levels of chemokines and immunosuppressive cytokines. Conversely, CD206−IM preferentially populate the alveolar interstitium and exhibit features of antigen-presenting cells. In addition, our data support that CD64+CD16.2+ monocytes arise from intravascular Ly-6Clo patrolling monocytes that enter the tissue at steady-state to become putative precursors of CD206−IM. This study expands our knowledge about the complexity of lung IM and reveals an ontogenic pathway for one IM subset, an important step for elaborating future macrophage-targeted therapies.

Suggested Citation

  • Joey Schyns & Qiang Bai & Cecilia Ruscitti & Coraline Radermecker & Sebastiaan Schepper & Svetoslav Chakarov & Frédéric Farnir & Dimitri Pirottin & Florent Ginhoux & Guy Boeckxstaens & Fabrice Bureau , 2019. "Non-classical tissue monocytes and two functionally distinct populations of interstitial macrophages populate the mouse lung," Nature Communications, Nature, vol. 10(1), pages 1-16, December.
  • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-11843-0
    DOI: 10.1038/s41467-019-11843-0
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    Cited by:

    1. Gan Zhao & Maria E. Gentile & Lulu Xue & Christopher V. Cosgriff & Aaron I. Weiner & Stephanie Adams-Tzivelekidis & Joanna Wong & Xinyuan Li & Sara Kass-Gergi & Nicolas P. Holcomb & Maria C. Basal & K, 2024. "Vascular endothelial-derived SPARCL1 exacerbates viral pneumonia through pro-inflammatory macrophage activation," Nature Communications, Nature, vol. 15(1), pages 1-19, December.
    2. Ning Yang & Joseph M. Luna & Peihong Dai & Yi Wang & Charles M. Rice & Liang Deng, 2022. "Lung type II alveolar epithelial cells collaborate with CCR2+ inflammatory monocytes in host defense against poxvirus infection," Nature Communications, Nature, vol. 13(1), pages 1-17, December.
    3. Amit A. Upadhyay & Elise G. Viox & Timothy N. Hoang & Arun K. Boddapati & Maria Pino & Michelle Y.-H. Lee & Jacqueline Corry & Zachary Strongin & David A. Cowan & Elizabeth N. Beagle & Tristan R. Hort, 2023. "TREM2+ and interstitial-like macrophages orchestrate airway inflammation in SARS-CoV-2 infection in rhesus macaques," Nature Communications, Nature, vol. 14(1), pages 1-16, December.

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