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Cell-type-specific genomics reveals histone modification dynamics in mammalian meiosis

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Listed:
  • Kwan-Wood Gabriel Lam

    (National Institutes of Health)

  • Kevin Brick

    (National Institutes of Health)

  • Gang Cheng

    (National Institutes of Health)

  • Florencia Pratto

    (National Institutes of Health)

  • R. Daniel Camerini-Otero

    (National Institutes of Health)

Abstract

Meiosis is the specialized cell division during which parental genomes recombine to create genotypically unique gametes. Despite its importance, mammalian meiosis cannot be studied in vitro, greatly limiting mechanistic studies. In vivo, meiocytes progress asynchronously through meiosis and therefore the study of specific stages of meiosis is a challenge. Here, we describe a method for isolating pure sub-populations of nuclei that allows for detailed study of meiotic substages. Interrogating the H3K4me3 landscape revealed dynamic chromatin transitions between substages of meiotic prophase I, both at sites of genetic recombination and at gene promoters. We also leveraged this method to perform the first comprehensive, genome-wide survey of histone marks in meiotic prophase, revealing a heretofore unappreciated complexity of the epigenetic landscape at meiotic recombination hotspots. Ultimately, this study presents a straightforward, scalable framework for interrogating the complexities of mammalian meiosis.

Suggested Citation

  • Kwan-Wood Gabriel Lam & Kevin Brick & Gang Cheng & Florencia Pratto & R. Daniel Camerini-Otero, 2019. "Cell-type-specific genomics reveals histone modification dynamics in mammalian meiosis," Nature Communications, Nature, vol. 10(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-11820-7
    DOI: 10.1038/s41467-019-11820-7
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