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RST1 and RIPR connect the cytosolic RNA exosome to the Ski complex in Arabidopsis

Author

Listed:
  • Heike Lange

    (Université de Strasbourg)

  • Simon Y. A. Ndecky

    (Université de Strasbourg)

  • Carlos Gomez-Diaz

    (Université de Strasbourg)

  • David Pflieger

    (Université de Strasbourg)

  • Nicolas Butel

    (Université Paris-Saclay)

  • Julie Zumsteg

    (Université de Strasbourg)

  • Lauriane Kuhn

    (Université de Strasbourg)

  • Christina Piermaria

    (Université de Strasbourg)

  • Johana Chicher

    (Université de Strasbourg)

  • Michael Christie

    (Max Planck Institute for Developmental Biology)

  • Ezgi S. Karaaslan

    (Max Planck Institute for Developmental Biology)

  • Patricia L. M. Lang

    (Max Planck Institute for Developmental Biology)

  • Detlef Weigel

    (Max Planck Institute for Developmental Biology)

  • Hervé Vaucheret

    (Université Paris-Saclay)

  • Philippe Hammann

    (Université de Strasbourg)

  • Dominique Gagliardi

    (Université de Strasbourg)

Abstract

The RNA exosome is a key 3’−5’ exoribonuclease with an evolutionarily conserved structure and function. Its cytosolic functions require the co-factors SKI7 and the Ski complex. Here we demonstrate by co-purification experiments that the ARM-repeat protein RESURRECTION1 (RST1) and RST1 INTERACTING PROTEIN (RIPR) connect the cytosolic Arabidopsis RNA exosome to the Ski complex. rst1 and ripr mutants accumulate RNA quality control siRNAs (rqc-siRNAs) produced by the post-transcriptional gene silencing (PTGS) machinery when mRNA degradation is compromised. The small RNA populations observed in rst1 and ripr mutants are also detected in mutants lacking the RRP45B/CER7 core exosome subunit. Thus, molecular and genetic evidence supports a physical and functional link between RST1, RIPR and the RNA exosome. Our data reveal the existence of additional cytosolic exosome co-factors besides the known Ski subunits. RST1 is not restricted to plants, as homologues with a similar domain architecture but unknown function exist in animals, including humans.

Suggested Citation

  • Heike Lange & Simon Y. A. Ndecky & Carlos Gomez-Diaz & David Pflieger & Nicolas Butel & Julie Zumsteg & Lauriane Kuhn & Christina Piermaria & Johana Chicher & Michael Christie & Ezgi S. Karaaslan & Pa, 2019. "RST1 and RIPR connect the cytosolic RNA exosome to the Ski complex in Arabidopsis," Nature Communications, Nature, vol. 10(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-11807-4
    DOI: 10.1038/s41467-019-11807-4
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