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Ca2+-regulated Ca2+ channels with an RCK gating ring control plant symbiotic associations

Author

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  • Sunghoon Kim

    (University of Texas Southwestern Medical Center
    University of Texas Southwestern Medical Center)

  • Weizhong Zeng

    (University of Texas Southwestern Medical Center
    University of Texas Southwestern Medical Center)

  • Shane Bernard

    (University of Wisconsin-Madison)

  • Jun Liao

    (ShanghaiTech University)

  • Muthusubramanian Venkateshwaran

    (University of Wisconsin-Platteville)

  • Jean-Michel Ane

    (University of Wisconsin-Madison)

  • Youxing Jiang

    (University of Texas Southwestern Medical Center
    University of Texas Southwestern Medical Center)

Abstract

A family of plant nuclear ion channels, including DMI1 (Does not Make Infections 1) and its homologs CASTOR and POLLUX, are required for the establishment of legume-microbe symbioses by generating nuclear and perinuclear Ca2+ spiking. Here we show that CASTOR from Lotus japonicus is a highly selective Ca2+ channel whose activation requires cytosolic/nucleosolic Ca2+, contrary to the previous suggestion of it being a K+ channel. Structurally, the cytosolic/nucleosolic ligand-binding soluble region of CASTOR contains two tandem RCK (Regulator of Conductance for K+) domains, and four subunits assemble into the gating ring architecture, similar to that of large conductance, Ca2+-gated K+ (BK) channels despite the lack of sequence similarity. Multiple ion binding sites are clustered at two locations within each subunit, and three of them are identified to be Ca2+ sites. Our in vitro and in vivo assays also demonstrate the importance of these gating-ring Ca2+ binding sites to the physiological function of CASTOR as well as DMI1.

Suggested Citation

  • Sunghoon Kim & Weizhong Zeng & Shane Bernard & Jun Liao & Muthusubramanian Venkateshwaran & Jean-Michel Ane & Youxing Jiang, 2019. "Ca2+-regulated Ca2+ channels with an RCK gating ring control plant symbiotic associations," Nature Communications, Nature, vol. 10(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-11698-5
    DOI: 10.1038/s41467-019-11698-5
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