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MICU1 controls cristae junction and spatially anchors mitochondrial Ca2+ uniporter complex

Author

Listed:
  • Benjamin Gottschalk

    (Medical University of Graz)

  • Christiane Klec

    (Medical University of Graz)

  • Gerd Leitinger

    (Medical University of Graz)

  • Eva Bernhart

    (Medical University of Graz)

  • René Rost

    (Medical University of Graz)

  • Helmut Bischof

    (Medical University of Graz)

  • Corina T. Madreiter-Sokolowski

    (Medical University of Graz)

  • Snježana Radulović

    (Medical University of Graz
    Medical University of Graz)

  • Emrah Eroglu

    (Medical University of Graz)

  • Wolfgang Sattler

    (Medical University of Graz
    BioTechMed Graz)

  • Markus Waldeck-Weiermair

    (Medical University of Graz)

  • Roland Malli

    (Medical University of Graz
    BioTechMed Graz)

  • Wolfgang F. Graier

    (Medical University of Graz
    BioTechMed Graz)

Abstract

Recently identified core proteins (MICU1, MCU, EMRE) forming the mitochondrial Ca2+ uniporter complex propelled investigations into its physiological workings. Here, we apply structured illumination microscopy to visualize and localize these proteins in living cells. Our data show that MICU1 localizes at the inner boundary membrane (IBM) due to electrostatic interaction of its polybasic domain. Moreover, this exclusive localization of MICU1 is important for the stability of cristae junctions (CJ), cytochrome c release and mitochondrial membrane potential. In contrast to MICU1, MCU and EMRE are homogeneously distributed at the inner mitochondrial membrane under resting conditions. However, upon Ca2+ elevation MCU and EMRE dynamically accumulate at the IBM in a MICU1-dependent manner. Eventually, our findings unveil an essential function of MICU1 in CJ stabilization and provide mechanistic insights of how sophistically MICU1 controls the MCU-Complex while maintaining the structural mitochondrial membrane framework.

Suggested Citation

  • Benjamin Gottschalk & Christiane Klec & Gerd Leitinger & Eva Bernhart & René Rost & Helmut Bischof & Corina T. Madreiter-Sokolowski & Snježana Radulović & Emrah Eroglu & Wolfgang Sattler & Markus Wald, 2019. "MICU1 controls cristae junction and spatially anchors mitochondrial Ca2+ uniporter complex," Nature Communications, Nature, vol. 10(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-11692-x
    DOI: 10.1038/s41467-019-11692-x
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