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Electrical pulse-induced electrochemical biosensor for hepatitis E virus detection

Author

Listed:
  • Ankan Dutta Chowdhury

    (Shizuoka University)

  • Kenshin Takemura

    (Shizuoka University)

  • Tian-Cheng Li

    (National Institute of Infectious Diseases)

  • Tetsuro Suzuki

    (Hamamatsu University of School of Medicine, Handayama)

  • Enoch Y. Park

    (Shizuoka University
    Shizuoka University)

Abstract

Hepatitis E virus (HEV) is one of the leading causes of acute viral hepatitis worldwide. In this work, a pulse-triggered ultrasensitive electrochemical sensor was fabricated using graphene quantum dots and gold-embedded polyaniline nanowires, prepared via an interfacial polymerization and then self-assembly approach. Introducing an external electrical pulse during the virus accumulation step increases the sensitivity towards HEV due to the expanded surface of the virus particle as well as the antibody-conjugated polyaniline chain length, compared to other conventional electrochemical sensors. The sensor was applied to various HEV genotypes, including G1, G3, G7 and ferret HEV obtained from cell culture supernatant and in a series of fecal specimen samples collected from G7 HEV-infected monkey. The sensitivity is similar to that detected by real-time quantitative reverse transcription-polymerase chain (RT-qPCR). These results suggests that the proposed sensor can pave the way for the development of robust, high-performance sensing methodologies for HEV detection.

Suggested Citation

  • Ankan Dutta Chowdhury & Kenshin Takemura & Tian-Cheng Li & Tetsuro Suzuki & Enoch Y. Park, 2019. "Electrical pulse-induced electrochemical biosensor for hepatitis E virus detection," Nature Communications, Nature, vol. 10(1), pages 1-12, December.
  • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-11644-5
    DOI: 10.1038/s41467-019-11644-5
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    Cited by:

    1. Kumar, Pradip & Dhand, Chetna & Dwivedi, Neeraj & Singh, Shiv & Khan, Raju & Verma, Sarika & Singh, Archana & Gupta, Manoj Kumar & Kumar, Surender & Kumar, Rajeev & Srivastava, Avanish Kumar, 2022. "Graphene quantum dots: A contemporary perspective on scope, opportunities, and sustainability," Renewable and Sustainable Energy Reviews, Elsevier, vol. 157(C).

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