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Dissecting the heterogeneity of DENV vaccine-elicited cellular immunity using single-cell RNA sequencing and metabolic profiling

Author

Listed:
  • Adam T. Waickman

    (Walter Reed Army Institute of Research)

  • Kaitlin Victor

    (Walter Reed Army Institute of Research)

  • Tao Li

    (Walter Reed Army Institute of Research)

  • Kristin Hatch

    (Walter Reed Army Institute of Research)

  • Wiriya Rutvisuttinunt

    (Walter Reed Army Institute of Research)

  • Carey Medin

    (University of Rhode Island)

  • Benjamin Gabriel

    (University of Rhode Island)

  • Richard G. Jarman

    (Walter Reed Army Institute of Research)

  • Heather Friberg

    (Walter Reed Army Institute of Research)

  • Jeffrey R. Currier

    (Walter Reed Army Institute of Research)

Abstract

Generating effective and durable T cell immunity is a critical prerequisite for vaccination against dengue virus (DENV) and other viral diseases. However, understanding the molecular mechanisms of vaccine-elicited T cell immunity remains a critical knowledge gap in vaccinology. In this study, we utilize single-cell RNA sequencing (scRNAseq) and longitudinal TCR clonotype analysis to identify a unique transcriptional signature present in acutely activated and clonally-expanded T cells that become committed to the memory repertoire. This effector/memory-associated transcriptional signature is dominated by a robust metabolic transcriptional program. Based on this transcriptional signature, we are able to define a set of markers that identify the most durable vaccine-reactive memory-precursor CD8+ T cells. This study illustrates the power of scRNAseq as an analytical tool to assess the molecular mechanisms of host control and vaccine modality in determining the magnitude, diversity and persistence of vaccine-elicited cell-mediated immunity.

Suggested Citation

  • Adam T. Waickman & Kaitlin Victor & Tao Li & Kristin Hatch & Wiriya Rutvisuttinunt & Carey Medin & Benjamin Gabriel & Richard G. Jarman & Heather Friberg & Jeffrey R. Currier, 2019. "Dissecting the heterogeneity of DENV vaccine-elicited cellular immunity using single-cell RNA sequencing and metabolic profiling," Nature Communications, Nature, vol. 10(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-11634-7
    DOI: 10.1038/s41467-019-11634-7
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    Cited by:

    1. Huirui Wang & Xiaona You & Jingcheng Wang & Xinyi Chen & Yinghui Gao & Mengmeng Wang & Wenru Zhang & Jiaozhen Zhang & Yang Yu & Bo Han & Mei Qi & Xiaohui Liu & Hongxiang Lou & Ting Dong, 2024. "MFSD7C protects hemolysis-induced lung impairments by inhibiting ferroptosis," Nature Communications, Nature, vol. 15(1), pages 1-17, December.
    2. Luisa Santus & Maria Sopena-Rios & Raquel García-Pérez & Aaron E. Lin & Gordon C. Adams & Kayla G. Barnes & Katherine J. Siddle & Shirlee Wohl & Ferran Reverter & John L. Rinn & Richard S. Bennett & L, 2023. "Single-cell profiling of lncRNA expression during Ebola virus infection in rhesus macaques," Nature Communications, Nature, vol. 14(1), pages 1-14, December.

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