IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v10y2019i1d10.1038_s41467-019-11604-z.html
   My bibliography  Save this article

Virus lasers for biological detection

Author

Listed:
  • John E. Hales

    (University College London)

  • Guy Matmon

    (Paul Scherrer Institut)

  • Paul A. Dalby

    (University College London)

  • John M. Ward

    (University College London)

  • Gabriel Aeppli

    (Paul Scherrer Institut
    ETH Zürich
    Institut de Physique, EPFL)

Abstract

The selective amplification of DNA in the polymerase chain reaction is used to exponentially increase the signal in molecular diagnostics for nucleic acids, but there are no analogous techniques for signal enhancement in clinical tests for proteins or cells. Instead, the signal from affinity-based measurements of these biomolecules depends linearly on the probe concentration. Substituting antibody-based probes tagged for fluorescent quantification with lasing detection probes would create a new platform for biomarker quantification based on optical rather than enzymatic amplification. Here, we construct a virus laser which bridges synthetic biology and laser physics, and demonstrate virus-lasing probes for biosensing. Our virus-lasing probes display an unprecedented > 10,000 times increase in signal from only a 50% increase in probe concentration, using fluorimeter-compatible optics, and can detect biomolecules at sub-100 fmol mL−1 concentrations.

Suggested Citation

  • John E. Hales & Guy Matmon & Paul A. Dalby & John M. Ward & Gabriel Aeppli, 2019. "Virus lasers for biological detection," Nature Communications, Nature, vol. 10(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-11604-z
    DOI: 10.1038/s41467-019-11604-z
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-019-11604-z
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-019-11604-z?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Ningning Tang & Jiadong Zhou & Liangxuan Wang & Matthias Stolte & Guojing Xie & Xinbo Wen & Linlin Liu & Frank Würthner & Johannes Gierschner & Zengqi Xie, 2023. "Anomalous deep-red luminescence of perylene black analogues with strong π-π interactions," Nature Communications, Nature, vol. 14(1), pages 1-10, December.
    2. Kaicheng Deng & Yao Tang & Yan Xiao & Danni Zhong & Hua Zhang & Wen Fang & Liyin Shen & Zhaochuang Wang & Jiazhen Pan & Yuwen Lu & Changming Chen & Yun Gao & Qiao Jin & Lenan Zhuang & Hao Wan & Liujin, 2023. "A biodegradable, flexible photonic patch for in vivo phototherapy," Nature Communications, Nature, vol. 14(1), pages 1-15, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-11604-z. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.