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Profiling surface proteins on individual exosomes using a proximity barcoding assay

Author

Listed:
  • Di Wu

    (Uppsala University
    Stockholm University
    Vesicode AB)

  • Junhong Yan

    (Uppsala University)

  • Xia Shen

    (University of Edinburgh
    Karolinska Institutet
    Sun Yat-sen University)

  • Yu Sun

    (Uppsala University
    College of Life Sciences, South China Agricultural University)

  • Måns Thulin

    (Uppsala University
    University of Edinburgh)

  • Yanling Cai

    (The Second Peopleʹs Hospital of Shenzhen)

  • Lotta Wik

    (Uppsala University)

  • Qiujin Shen

    (Uppsala University)

  • Johan Oelrich

    (Vesicode AB)

  • Xiaoyan Qian

    (Stockholm University)

  • K. Louise Dubois

    (Uppsala University)

  • K. Göran Ronquist

    (Uppsala University)

  • Mats Nilsson

    (Stockholm University)

  • Ulf Landegren

    (Uppsala University)

  • Masood Kamali-Moghaddam

    (Uppsala University)

Abstract

Exosomes have been implicated in numerous biological processes, and they may serve as important disease markers. Surface proteins on exosomes carry information about their tissues of origin. Because of the heterogeneity of exosomes it is desirable to investigate them individually, but this has so far remained impractical. Here, we demonstrate a proximity-dependent barcoding assay to profile surface proteins of individual exosomes using antibody-DNA conjugates and next-generation sequencing. We first validate the method using artificial streptavidin-oligonucleotide complexes, followed by analysis of the variable composition of surface proteins on individual exosomes, derived from human body fluids or cell culture media. Exosomes from different sources are characterized by the presence of specific combinations of surface proteins and their abundance, allowing exosomes to be separately quantified in mixed samples to serve as markers for tissue-specific engagement in disease.

Suggested Citation

  • Di Wu & Junhong Yan & Xia Shen & Yu Sun & Måns Thulin & Yanling Cai & Lotta Wik & Qiujin Shen & Johan Oelrich & Xiaoyan Qian & K. Louise Dubois & K. Göran Ronquist & Mats Nilsson & Ulf Landegren & Mas, 2019. "Profiling surface proteins on individual exosomes using a proximity barcoding assay," Nature Communications, Nature, vol. 10(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-11486-1
    DOI: 10.1038/s41467-019-11486-1
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