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The MTR4 helicase recruits nuclear adaptors of the human RNA exosome using distinct arch-interacting motifs

Author

Listed:
  • Mahesh Lingaraju

    (Max-Planck-Institute of Biochemistry)

  • Dennis Johnsen

    (Aarhus University)

  • Andreas Schlundt

    (Technical University of Munich (TUM)
    Helmholtz-Zentrum München
    Institute for Molecular Biosciences and Center for Biomolecular Magnetic Resonance (BMRZ) at Johann Wolfgang Goethe-University)

  • Lukas M. Langer

    (Max-Planck-Institute of Biochemistry)

  • Jérôme Basquin

    (Max-Planck-Institute of Biochemistry)

  • Michael Sattler

    (Technical University of Munich (TUM)
    Helmholtz-Zentrum München)

  • Torben Heick Jensen

    (Aarhus University)

  • Sebastian Falk

    (Max-Planck-Institute of Biochemistry
    University of Vienna)

  • Elena Conti

    (Max-Planck-Institute of Biochemistry)

Abstract

The nuclear exosome and its essential co-factor, the RNA helicase MTR4, play crucial roles in several RNA degradation pathways. Besides unwinding RNA substrates for exosome-mediated degradation, MTR4 associates with RNA-binding proteins that function as adaptors in different RNA processing and decay pathways. Here, we identify and characterize the interactions of human MTR4 with a ribosome processing adaptor, NVL, and with ZCCHC8, an adaptor involved in the decay of small nuclear RNAs. We show that the unstructured regions of NVL and ZCCHC8 contain short linear motifs that bind the MTR4 arch domain in a mutually exclusive manner. These short sequences diverged from the arch-interacting motif (AIM) of yeast rRNA processing factors. Our results suggest that nuclear exosome adaptors have evolved canonical and non-canonical AIM sequences to target human MTR4 and demonstrate the versatility and specificity with which the MTR4 arch domain can recruit a repertoire of different RNA-binding proteins.

Suggested Citation

  • Mahesh Lingaraju & Dennis Johnsen & Andreas Schlundt & Lukas M. Langer & Jérôme Basquin & Michael Sattler & Torben Heick Jensen & Sebastian Falk & Elena Conti, 2019. "The MTR4 helicase recruits nuclear adaptors of the human RNA exosome using distinct arch-interacting motifs," Nature Communications, Nature, vol. 10(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-11339-x
    DOI: 10.1038/s41467-019-11339-x
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