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The late stage of COPI vesicle fission requires shorter forms of phosphatidic acid and diacylglycerol

Author

Listed:
  • Seung-Yeol Park

    (Harvard Medical School
    Harvard Medical School
    Pohang University of Science and Technology)

  • Jia-Shu Yang

    (Harvard Medical School
    Harvard Medical School)

  • Zhen Li

    (City University of Hong Kong)

  • Pan Deng

    (University of Kentucky
    Lexington Veterans Affairs Medical Center)

  • Xiaohong Zhu

    (City University of Hong Kong)

  • David Young

    (Harvard Medical School
    Harvard Medical School)

  • Maria Ericsson

    (Harvard Medical School)

  • Ruben L. H. Andringa

    (University of Groningen)

  • Adriaan J. Minnaard

    (University of Groningen)

  • Chunmei Zhu

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Fei Sun

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences
    Chinese Academy of Sciences)

  • D. Branch Moody

    (Harvard Medical School
    Harvard Medical School)

  • Andrew J. Morris

    (University of Kentucky
    Lexington Veterans Affairs Medical Center)

  • Jun Fan

    (City University of Hong Kong
    Shenzhen Research Institute)

  • Victor W. Hsu

    (Harvard Medical School
    Harvard Medical School)

Abstract

Studies on vesicle formation by the Coat Protein I (COPI) complex have contributed to a basic understanding of how vesicular transport is initiated. Phosphatidic acid (PA) and diacylglycerol (DAG) have been found previously to be required for the fission stage of COPI vesicle formation. Here, we find that PA with varying lipid geometry can all promote early fission, but only PA with shortened acyl chains promotes late fission. Moreover, diacylglycerol (DAG) acts after PA in late fission, with this role of DAG also requiring shorter acyl chains. Further highlighting the importance of the short-chain lipid geometry for late fission, we find that shorter forms of PA and DAG promote the vesiculation ability of COPI fission factors. These findings advance a general understanding of how lipid geometry contributes to membrane deformation for vesicle fission, and also how proteins and lipids coordinate their actions in driving this process.

Suggested Citation

  • Seung-Yeol Park & Jia-Shu Yang & Zhen Li & Pan Deng & Xiaohong Zhu & David Young & Maria Ericsson & Ruben L. H. Andringa & Adriaan J. Minnaard & Chunmei Zhu & Fei Sun & D. Branch Moody & Andrew J. Mor, 2019. "The late stage of COPI vesicle fission requires shorter forms of phosphatidic acid and diacylglycerol," Nature Communications, Nature, vol. 10(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-11324-4
    DOI: 10.1038/s41467-019-11324-4
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