IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v10y2019i1d10.1038_s41467-019-11289-4.html
   My bibliography  Save this article

Targeting metabolic driving and intermediate influx in lysine catabolism for high-level glutarate production

Author

Listed:
  • Wenna Li

    (Beijing University of Chemical Technology)

  • Lin Ma

    (Beijing University of Chemical Technology)

  • Xiaolin Shen

    (Beijing University of Chemical Technology)

  • Jia Wang

    (Beijing University of Chemical Technology)

  • Qi Feng

    (Beijing University of Chemical Technology)

  • Lexuan Liu

    (Beijing University of Chemical Technology)

  • Guojun Zheng

    (Beijing University of Chemical Technology)

  • Yajun Yan

    (The University of Georgia)

  • Xinxiao Sun

    (Beijing University of Chemical Technology)

  • Qipeng Yuan

    (Beijing University of Chemical Technology)

Abstract

Various biosynthetic pathways have been designed to explore sustainable production of glutarate, an attractive C5 building block of polyesters and polyamides. However, its efficient production has not been achieved in Escherichia coli. Here, we use E. coli native lysine catabolic machinery for glutarate biosynthesis. This endogenous genes-only design can generate strong metabolic driving force to maximize carbon flux toward glutarate biosynthesis by replenishing glutamate and NAD(P)H for lysine biosynthesis, releasing lysine feedback inhibition, and boosting oxaloacetate supply. We use native transporters to overcome extracellular accumulation of cadaverine and 5-aminovalerate. With these efforts, both high titer (54.5 g L−1) and high yield (0.54 mol mol−1 glucose) of glutarate production are achieved under fed-batch conditions. This work demonstrates the power of redirecting carbon flux and the role of transporters to decrease intermediate accumulation.

Suggested Citation

  • Wenna Li & Lin Ma & Xiaolin Shen & Jia Wang & Qi Feng & Lexuan Liu & Guojun Zheng & Yajun Yan & Xinxiao Sun & Qipeng Yuan, 2019. "Targeting metabolic driving and intermediate influx in lysine catabolism for high-level glutarate production," Nature Communications, Nature, vol. 10(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-11289-4
    DOI: 10.1038/s41467-019-11289-4
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-019-11289-4
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-019-11289-4?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Zhilan Zhang & Ruyin Chu & Wanqing Wei & Wei Song & Chao Ye & Xiulai Chen & Jing Wu & Liming Liu & Cong Gao, 2024. "RETRACTED ARTICLE: Systems engineering of Escherichia coli for high-level glutarate production from glucose," Nature Communications, Nature, vol. 15(1), pages 1-14, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-11289-4. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.