IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v10y2019i1d10.1038_s41467-019-11179-9.html
   My bibliography  Save this article

Genome-wide systematic identification of methyltransferase recognition and modification patterns

Author

Listed:
  • Torbjørn Ølshøj Jensen

    (Technical University of Denmark (DTU))

  • Christian Tellgren-Roth

    (National Genomics Infrastructure, SciLifeLab)

  • Stephanie Redl

    (Technical University of Denmark (DTU)
    Massachusetts Institute of Technology)

  • Jérôme Maury

    (Technical University of Denmark (DTU))

  • Simo Abdessamad Baallal Jacobsen

    (Technical University of Denmark (DTU))

  • Lasse Ebdrup Pedersen

    (Technical University of Denmark (DTU))

  • Alex Toftgaard Nielsen

    (Technical University of Denmark (DTU))

Abstract

Genome-wide analysis of DNA methylation patterns using single molecule real-time DNA sequencing has boosted the number of publicly available methylomes. However, there is a lack of tools coupling methylation patterns and the corresponding methyltransferase genes. Here we demonstrate a high-throughput method for coupling methyltransferases with their respective motifs, using automated cloning and analysing the methyltransferases in vectors carrying a strain-specific cassette containing all potential target sites. To validate the method, we analyse the genomes of the thermophile Moorella thermoacetica and the mesophile Acetobacterium woodii, two acetogenic bacteria having substantially modified genomes with 12 methylation motifs and a total of 23 methyltransferase genes. Using our method, we characterize the 23 methyltransferases, assign motifs to the respective enzymes and verify activity for 11 of the 12 motifs.

Suggested Citation

  • Torbjørn Ølshøj Jensen & Christian Tellgren-Roth & Stephanie Redl & Jérôme Maury & Simo Abdessamad Baallal Jacobsen & Lasse Ebdrup Pedersen & Alex Toftgaard Nielsen, 2019. "Genome-wide systematic identification of methyltransferase recognition and modification patterns," Nature Communications, Nature, vol. 10(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-11179-9
    DOI: 10.1038/s41467-019-11179-9
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-019-11179-9
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-019-11179-9?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-11179-9. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.