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Cul3 and insomniac are required for rapid ubiquitination of postsynaptic targets and retrograde homeostatic signaling

Author

Listed:
  • Koto Kikuma

    (University of Southern California)

  • Xiling Li

    (University of Southern California)

  • Sarah Perry

    (University of Southern California)

  • Qiuling Li

    (NYU School of Medicine)

  • Pragya Goel

    (University of Southern California)

  • Catherine Chen

    (University of Southern California)

  • Daniel Kim

    (University of Southern California)

  • Nicholas Stavropoulos

    (NYU School of Medicine)

  • Dion Dickman

    (University of Southern California)

Abstract

At the Drosophila neuromuscular junction, inhibition of postsynaptic glutamate receptors activates retrograde signaling that precisely increases presynaptic neurotransmitter release to restore baseline synaptic strength. However, the nature of the underlying postsynaptic induction process remains enigmatic. Here, we design a forward genetic screen to discover factors in the postsynaptic compartment necessary to generate retrograde homeostatic signaling. This approach identified insomniac (inc), a putative adaptor for the Cullin-3 (Cul3) ubiquitin ligase complex, which together with Cul3 is essential for normal sleep regulation. Interestingly, we find that Inc and Cul3 rapidly accumulate at postsynaptic compartments following acute receptor inhibition and are required for a local increase in mono-ubiquitination. Finally, we show that Peflin, a Ca2+-regulated Cul3 co-adaptor, is necessary for homeostatic communication, suggesting a relationship between Ca2+ signaling and control of Cul3/Inc activity in the postsynaptic compartment. Our study suggests that Cul3/Inc-dependent mono-ubiquitination, compartmentalized at postsynaptic densities, gates retrograde signaling and provides an intriguing molecular link between the control of sleep and homeostatic plasticity at synapses.

Suggested Citation

  • Koto Kikuma & Xiling Li & Sarah Perry & Qiuling Li & Pragya Goel & Catherine Chen & Daniel Kim & Nicholas Stavropoulos & Dion Dickman, 2019. "Cul3 and insomniac are required for rapid ubiquitination of postsynaptic targets and retrograde homeostatic signaling," Nature Communications, Nature, vol. 10(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-10992-6
    DOI: 10.1038/s41467-019-10992-6
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    Cited by:

    1. Sarah Perry & Yifu Han & Chengjie Qiu & Chun Chien & Pragya Goel & Samantha Nishimura & Manisha Sajnani & Andreas Schmid & Stephan J. Sigrist & Dion Dickman, 2022. "A glutamate receptor C-tail recruits CaMKII to suppress retrograde homeostatic signaling," Nature Communications, Nature, vol. 13(1), pages 1-16, December.

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