IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v10y2019i1d10.1038_s41467-019-10806-9.html
   My bibliography  Save this article

Structural basis for the homotypic fusion of chlamydial inclusions by the SNARE-like protein IncA

Author

Listed:
  • Gino Cingolani

    (Thomas Jefferson University, Department of Biochemistry and Molecular Biology
    Institute of Biomembranes and Bioenergetics, National Research Council)

  • Michael McCauley

    (Thomas Jefferson University, Department of Microbiology and Immunology
    Janssen Research and Development)

  • Anna Lobley

    (Thomas Jefferson University, Department of Microbiology and Immunology)

  • Alexander J. Bryer

    (The University of Delaware, Department of Chemistry and Biochemistry)

  • Jordan Wesolowski

    (Thomas Jefferson University, Department of Microbiology and Immunology)

  • Deanna L. Greco

    (The University of Delaware, Department of Chemistry and Biochemistry)

  • Ravi K. Lokareddy

    (Thomas Jefferson University, Department of Biochemistry and Molecular Biology)

  • Erik Ronzone

    (Thomas Jefferson University, Department of Microbiology and Immunology
    VUE Health)

  • Juan R. Perilla

    (The University of Delaware, Department of Chemistry and Biochemistry)

  • Fabienne Paumet

    (Thomas Jefferson University, Department of Microbiology and Immunology)

Abstract

Many intracellular bacteria, including Chlamydia, establish a parasitic membrane-bound organelle inside the host cell that is essential for the bacteria’s survival. Chlamydia trachomatis forms inclusions that are decorated with poorly characterized membrane proteins known as Incs. The prototypical Inc, called IncA, enhances Chlamydia pathogenicity by promoting the homotypic fusion of inclusions and shares structural and functional similarity to eukaryotic SNAREs. Here, we present the atomic structure of the cytoplasmic domain of IncA, which reveals a non-canonical four-helix bundle. Structure-based mutagenesis, molecular dynamics simulation, and functional cellular assays identify an intramolecular clamp that is essential for IncA-mediated homotypic membrane fusion during infection.

Suggested Citation

  • Gino Cingolani & Michael McCauley & Anna Lobley & Alexander J. Bryer & Jordan Wesolowski & Deanna L. Greco & Ravi K. Lokareddy & Erik Ronzone & Juan R. Perilla & Fabienne Paumet, 2019. "Structural basis for the homotypic fusion of chlamydial inclusions by the SNARE-like protein IncA," Nature Communications, Nature, vol. 10(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-10806-9
    DOI: 10.1038/s41467-019-10806-9
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-019-10806-9
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-019-10806-9?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Christine Linton & Jordan Wesolowski & Anna Lobley & Toshiyuki Yamaji & Kentaro Hanada & Fabienne Paumet, 2024. "Specialized contact sites regulate the fusion of chlamydial inclusion membranes," Nature Communications, Nature, vol. 15(1), pages 1-15, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-10806-9. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.