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Site-specific cleavage of bacterial MucD by secreted proteases mediates antibacterial resistance in Arabidopsis

Author

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  • Yiming Wang

    (Max Planck Institute for Plant Breeding Research)

  • Ruben Garrido-Oter

    (Max Planck Institute for Plant Breeding Research
    Heinrich Heine University Düsseldorf)

  • Jingni Wu

    (Max Planck Institute for Plant Breeding Research
    Chinese Academy of Sciences)

  • Thomas M. Winkelmüller

    (Max Planck Institute for Plant Breeding Research)

  • Matthew Agler

    (Max Planck Institute for Plant Breeding Research
    Friedrich-Schiller University Jena)

  • Thomas Colby

    (Max Planck Institute for Plant Breeding Research
    Max Planck Institute for Biology of Ageing)

  • Tatsuya Nobori

    (Max Planck Institute for Plant Breeding Research)

  • Eric Kemen

    (Max Planck Institute for Plant Breeding Research
    University of Tübingen)

  • Kenichi Tsuda

    (Max Planck Institute for Plant Breeding Research)

Abstract

Plant innate immunity restricts growth of bacterial pathogens that threaten global food security. However, the mechanisms by which plant immunity suppresses bacterial growth remain enigmatic. Here we show that Arabidopsis thaliana secreted aspartic protease 1 and 2 (SAP1 and SAP2) cleave the evolutionarily conserved bacterial protein MucD to redundantly inhibit the growth of the bacterial pathogen Pseudomonas syringae. Antibacterial activity of SAP1 requires its protease activity in planta and in vitro. Plants overexpressing SAP1 exhibit enhanced MucD cleavage and resistance but incur no penalties in growth and reproduction, while sap1 sap2 double mutant plants exhibit compromised MucD cleavage and resistance against P. syringae. P. syringae lacking mucD shows compromised growth in planta and in vitro. Notably, growth of ΔmucD complemented with the non-cleavable MucDF106Y is not affected by SAP activity in planta and in vitro. Our findings identify the genetic factors and biochemical process underlying an antibacterial mechanism in plants.

Suggested Citation

  • Yiming Wang & Ruben Garrido-Oter & Jingni Wu & Thomas M. Winkelmüller & Matthew Agler & Thomas Colby & Tatsuya Nobori & Eric Kemen & Kenichi Tsuda, 2019. "Site-specific cleavage of bacterial MucD by secreted proteases mediates antibacterial resistance in Arabidopsis," Nature Communications, Nature, vol. 10(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-10793-x
    DOI: 10.1038/s41467-019-10793-x
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