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Urinary tract colonization is enhanced by a plasmid that regulates uropathogenic Acinetobacter baumannii chromosomal genes

Author

Listed:
  • Gisela Di Venanzio

    (Washington University School of Medicine)

  • Ana L. Flores-Mireles

    (Washington University School of Medicine
    University of Notre Dame)

  • Juan J. Calix

    (Washington University School of Medicine)

  • M. Florencia Haurat

    (Washington University School of Medicine)

  • Nichollas E. Scott

    (University of Melbourne at the Peter Doherty)

  • Lauren D. Palmer

    (Vanderbilt University Medical Center)

  • Robert F. Potter

    (Washington University School of Medicine in St. Louis)

  • Michael E. Hibbing

    (Washington University School of Medicine)

  • Laura Friedman

    (Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Departamento de Microbiología, Inmunología, Biotecnología y Genética, Cátedra de Microbiología)

  • Bin Wang

    (Washington University School of Medicine in St. Louis)

  • Gautam Dantas

    (Washington University School of Medicine
    Washington University School of Medicine in St. Louis
    Washington University School of Medicine
    Washington University in St. Louis)

  • Eric P. Skaar

    (Vanderbilt University Medical Center)

  • Scott J. Hultgren

    (Washington University School of Medicine)

  • Mario F. Feldman

    (Washington University School of Medicine)

Abstract

Multidrug resistant (MDR) Acinetobacter baumannii poses a growing threat to global health. Research on Acinetobacter pathogenesis has primarily focused on pneumonia and bloodstream infections, even though one in five A. baumannii strains are isolated from urinary sites. In this study, we highlight the role of A. baumannii as a uropathogen. We develop the first A. baumannii catheter-associated urinary tract infection (CAUTI) murine model using UPAB1, a recent MDR urinary isolate. UPAB1 carries the plasmid pAB5, a member of the family of large conjugative plasmids that represses the type VI secretion system (T6SS) in multiple Acinetobacter strains. pAB5 confers niche specificity, as its carriage improves UPAB1 survival in a CAUTI model and decreases virulence in a pneumonia model. Comparative proteomic and transcriptomic analyses show that pAB5 regulates the expression of multiple chromosomally-encoded virulence factors besides T6SS. Our results demonstrate that plasmids can impact bacterial infections by controlling the expression of chromosomal genes.

Suggested Citation

  • Gisela Di Venanzio & Ana L. Flores-Mireles & Juan J. Calix & M. Florencia Haurat & Nichollas E. Scott & Lauren D. Palmer & Robert F. Potter & Michael E. Hibbing & Laura Friedman & Bin Wang & Gautam Da, 2019. "Urinary tract colonization is enhanced by a plasmid that regulates uropathogenic Acinetobacter baumannii chromosomal genes," Nature Communications, Nature, vol. 10(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-10706-y
    DOI: 10.1038/s41467-019-10706-y
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    Cited by:

    1. Laura Toribio-Celestino & Alicia Calvo-Villamañán & Cristina Herencias & Aida Alonso-del Valle & Jorge Sastre-Dominguez & Susana Quesada & Didier Mazel & Eduardo P. C. Rocha & Ariadna Fernández-Calvet, 2024. "A plasmid-chromosome crosstalk in multidrug resistant enterobacteria," Nature Communications, Nature, vol. 15(1), pages 1-17, December.
    2. Jonathan J. Molina & Kurt N. Kohler & Christopher Gager & Marissa J. Andersen & Ellsa Wongso & Elizabeth R. Lucas & Andrew Paik & Wei Xu & Deborah L. Donahue & Karla Bergeron & Aleksandra Klim & Micha, 2024. "Fibrinolytic-deficiencies predispose hosts to septicemia from a catheter-associated UTI," Nature Communications, Nature, vol. 15(1), pages 1-16, December.

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