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Conformational proofreading of distant 40S ribosomal subunit maturation events by a long-range communication mechanism

Author

Listed:
  • Valentin Mitterer

    (University of Graz
    University of Heidelberg)

  • Ramtin Shayan

    (Centre de Biologie Intégrative, Université de Toulouse, CNRS, UPS)

  • Sébastien Ferreira-Cerca

    (Genetics and Microbiology, University of Regensburg)

  • Guillaume Murat

    (University of Fribourg)

  • Tanja Enne

    (University of Graz)

  • Dana Rinaldi

    (Centre de Biologie Intégrative, Université de Toulouse, CNRS, UPS)

  • Sarah Weigl

    (University of Graz)

  • Hajrija Omanic

    (University of Graz)

  • Pierre-Emmanuel Gleizes

    (Centre de Biologie Intégrative, Université de Toulouse, CNRS, UPS)

  • Dieter Kressler

    (University of Fribourg)

  • Celia Plisson-Chastang

    (Centre de Biologie Intégrative, Université de Toulouse, CNRS, UPS)

  • Brigitte Pertschy

    (University of Graz)

Abstract

Eukaryotic ribosomes are synthesized in a hierarchical process driven by a plethora of assembly factors, but how maturation events at physically distant sites on pre-ribosomes are coordinated is poorly understood. Using functional analyses and cryo-EM, we show that ribosomal protein Rps20 orchestrates communication between two multi-step maturation events across the pre-40S subunit. Our study reveals that during pre-40S maturation, formation of essential contacts between Rps20 and Rps3 permits assembly factor Ltv1 to recruit the Hrr25 kinase, thereby promoting Ltv1 phosphorylation. In parallel, a deeply buried Rps20 loop reaches to the opposite pre-40S side, where it stimulates Rio2 ATPase activity. Both cascades converge to the final maturation steps releasing Rio2 and phosphorylated Ltv1. We propose that conformational proofreading exerted via Rps20 constitutes a checkpoint permitting assembly factor release and progression of pre-40S maturation only after completion of all earlier maturation steps.

Suggested Citation

  • Valentin Mitterer & Ramtin Shayan & Sébastien Ferreira-Cerca & Guillaume Murat & Tanja Enne & Dana Rinaldi & Sarah Weigl & Hajrija Omanic & Pierre-Emmanuel Gleizes & Dieter Kressler & Celia Plisson-Ch, 2019. "Conformational proofreading of distant 40S ribosomal subunit maturation events by a long-range communication mechanism," Nature Communications, Nature, vol. 10(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-10678-z
    DOI: 10.1038/s41467-019-10678-z
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    Cited by:

    1. Ken Ikeuchi & Nives Ivic & Robert Buschauer & Jingdong Cheng & Thomas Fröhlich & Yoshitaka Matsuo & Otto Berninghausen & Toshifumi Inada & Thomas Becker & Roland Beckmann, 2023. "Molecular basis for recognition and deubiquitination of 40S ribosomes by Otu2," Nature Communications, Nature, vol. 14(1), pages 1-15, December.

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