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Addendum: Precise tuning of gene expression levels in mammalian cells

Author

Listed:
  • Yale S. Michaels

    (University of Oxford)

  • Mike B. Barnkob

    (Weatherall Institute of Molecular Medicine University of Oxford)

  • Hector Barbosa

    (University of Oxford)

  • Toni A. Baeumler

    (University of Oxford)

  • Mary K. Thompson

    (University of Oxford)

  • Violaine Andre

    (Weatherall Institute of Molecular Medicine University of Oxford)

  • Huw Colin-York

    (Weatherall Institute of Molecular Medicine University of Oxford)

  • Marco Fritzsche

    (Weatherall Institute of Molecular Medicine University of Oxford
    Rheumatology and Musculoskeletal Sciences)

  • Uzi Gileadi

    (Weatherall Institute of Molecular Medicine University of Oxford)

  • Hilary M. Sheppard

    (University of Auckland)

  • David J. H. F. Knapp

    (University of Oxford)

  • Thomas A. Milne

    (University of Oxford)

  • Vincenzo Cerundolo

    (Weatherall Institute of Molecular Medicine University of Oxford)

  • Tudor A. Fulga

    (University of Oxford)

Abstract

Following re-sequencing of the miSFIT constructs used in the paper, two of the construct variants inserted into the 3’UTR of PD-1, namely ‘12C’ and ‘17A, 18G’, have been found to contain additional insertions not present in the other construct variants. The data points corresponding to these constructs in Figs. 2c, f and Supplementary Fig. 9 are therefore no longer valid. However the overall conclusion that step-wise control over gene expression levels using the miSFIT constructs remains unaffected by these errors. Updated versions of Fig. 2 and Supplementary Fig. 9 are presented in the accompanying Addendum.

Suggested Citation

  • Yale S. Michaels & Mike B. Barnkob & Hector Barbosa & Toni A. Baeumler & Mary K. Thompson & Violaine Andre & Huw Colin-York & Marco Fritzsche & Uzi Gileadi & Hilary M. Sheppard & David J. H. F. Knapp , 2019. "Addendum: Precise tuning of gene expression levels in mammalian cells," Nature Communications, Nature, vol. 10(1), pages 1-3, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-10615-0
    DOI: 10.1038/s41467-019-10615-0
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