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Structure and function of the Orc1 BAH-nucleosome complex

Author

Listed:
  • Pablo De Ioannes

    (New York University School of Medicine)

  • Victor A. Leon

    (New York University)

  • Zheng Kuang

    (NYU Langone Health
    University of Texas Southwestern Medical Center)

  • Miao Wang

    (New York University School of Medicine)

  • Jef D. Boeke

    (NYU Langone Health)

  • Andreas Hochwagen

    (New York University)

  • Karim-Jean Armache

    (New York University School of Medicine)

Abstract

The Origin Recognition Complex (ORC) is essential for replication, heterochromatin formation, telomere maintenance and genome stability in eukaryotes. Here we present the structure of the yeast Orc1 BAH domain bound to the nucleosome core particle. Our data reveal that Orc1, unlike its close homolog Sir3 involved in gene silencing, does not appear to discriminate between acetylated and non-acetylated lysine 16, modification states of the histone H4 tail that specify open and closed chromatin respectively. We elucidate the mechanism for this unique feature of Orc1 and hypothesize that its ability to interact with nucleosomes regardless of K16 modification state enables it to perform critical functions in both hetero- and euchromatin. We also show that direct interactions with nucleosomes are essential for Orc1 to maintain the integrity of rDNA borders during meiosis, a process distinct and independent from its known roles in silencing and replication.

Suggested Citation

  • Pablo De Ioannes & Victor A. Leon & Zheng Kuang & Miao Wang & Jef D. Boeke & Andreas Hochwagen & Karim-Jean Armache, 2019. "Structure and function of the Orc1 BAH-nucleosome complex," Nature Communications, Nature, vol. 10(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-10609-y
    DOI: 10.1038/s41467-019-10609-y
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    Cited by:

    1. Humberto Sánchez & Zhaowei Liu & Edo Veen & Theo Laar & John F. X. Diffley & Nynke H. Dekker, 2023. "A chromatinized origin reduces the mobility of ORC and MCM through interactions and spatial constraint," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    2. Sai Li & Michael R. Wasserman & Olga Yurieva & Lu Bai & Michael E. O’Donnell & Shixin Liu, 2022. "Nucleosome-directed replication origin licensing independent of a consensus DNA sequence," Nature Communications, Nature, vol. 13(1), pages 1-15, December.

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