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Regulating intracellular fate of siRNA by endoplasmic reticulum membrane-decorated hybrid nanoplexes

Author

Listed:
  • Chong Qiu

    (Peking University)

  • Hu-Hu Han

    (Peking University)

  • Jing Sun

    (Peking University)

  • Hai-Tao Zhang

    (Central South University)

  • Wei Wei

    (Peking University)

  • Shi-He Cui

    (Peking University)

  • Xin Chen

    (Peking University)

  • Jian-Cheng Wang

    (Peking University)

  • Qiang Zhang

    (Peking University)

Abstract

Most cationic vectors are difficult to avoid the fate of small interfering RNA (siRNA) degradation following the endosome-lysosome pathway during siRNA transfection. In this study, the endoplasmic reticulum (ER) membrane isolated from cancer cells was used to fabricate an integrative hybrid nanoplexes (EhCv/siRNA NPs) for improving siRNA transfection. Compared to the undecorated Cv/siEGFR NPs, the ER membrane-decorated EhCv/siRNA NPs exhibits a significantly higher gene silencing effect of siRNA in vitro and a better antitumor activity in nude mice bearing MCF-7 human breast tumor in vivo. Further mechanistic studies demonstrate that functional proteins on the ER membrane plays important roles on improving cellular uptake and altering intracellular trafficking pathway of siRNA. It is worth to believe that the ER membrane decoration on nanoplexes can effectively transport siRNA through the endosome-Golgi-ER pathway to evade lysosomal degradation and enhance the silencing effects of siRNA.

Suggested Citation

  • Chong Qiu & Hu-Hu Han & Jing Sun & Hai-Tao Zhang & Wei Wei & Shi-He Cui & Xin Chen & Jian-Cheng Wang & Qiang Zhang, 2019. "Regulating intracellular fate of siRNA by endoplasmic reticulum membrane-decorated hybrid nanoplexes," Nature Communications, Nature, vol. 10(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-10562-w
    DOI: 10.1038/s41467-019-10562-w
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    Cited by:

    1. Yan Zou & Yajing Sun & Yibin Wang & Dongya Zhang & Huiqing Yang & Xin Wang & Meng Zheng & Bingyang Shi, 2023. "Cancer cell-mitochondria hybrid membrane coated Gboxin loaded nanomedicines for glioblastoma treatment," Nature Communications, Nature, vol. 14(1), pages 1-19, December.

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