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Multi-immersion open-top light-sheet microscope for high-throughput imaging of cleared tissues

Author

Listed:
  • Adam K. Glaser

    (University of Washington)

  • Nicholas P. Reder

    (University of Washington)

  • Ye Chen

    (University of Washington)

  • Chengbo Yin

    (University of Washington)

  • Linpeng Wei

    (University of Washington)

  • Soyoung Kang

    (University of Washington)

  • Lindsey A. Barner

    (University of Washington)

  • Weisi Xie

    (University of Washington)

  • Erin F. McCarty

    (University of Washington)

  • Chenyi Mao

    (University of Washington)

  • Aaron R. Halpern

    (University of Washington)

  • Caleb R. Stoltzfus

    (University of Washington)

  • Jonathan S. Daniels

    (Applied Scientific Instrumentation)

  • Michael Y. Gerner

    (University of Washington)

  • Philip R. Nicovich

    (Allen Institute for Brain Science)

  • Joshua C. Vaughan

    (University of Washington
    University of Washington)

  • Lawrence D. True

    (University of Washington)

  • Jonathan T. C. Liu

    (University of Washington
    University of Washington)

Abstract

Recent advances in optical clearing and light-sheet microscopy have provided unprecedented access to structural and molecular information from intact tissues. However, current light-sheet microscopes have imposed constraints on the size, shape, number of specimens, and compatibility with various clearing protocols. Here we present a multi-immersion open-top light-sheet microscope that enables simple mounting of multiple specimens processed with a variety of clearing protocols, which will facilitate wide adoption by preclinical researchers and clinical laboratories. In particular, the open-top geometry provides unsurpassed versatility to interface with a wide range of accessory technologies in the future.

Suggested Citation

  • Adam K. Glaser & Nicholas P. Reder & Ye Chen & Chengbo Yin & Linpeng Wei & Soyoung Kang & Lindsey A. Barner & Weisi Xie & Erin F. McCarty & Chenyi Mao & Aaron R. Halpern & Caleb R. Stoltzfus & Jonatha, 2019. "Multi-immersion open-top light-sheet microscope for high-throughput imaging of cleared tissues," Nature Communications, Nature, vol. 10(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-10534-0
    DOI: 10.1038/s41467-019-10534-0
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    Cited by:

    1. Nikita Vladimirov & Fabian F. Voigt & Thomas Naert & Gabriela R. Araujo & Ruiyao Cai & Anna Maria Reuss & Shan Zhao & Patricia Schmid & Sven Hildebrand & Martina Schaettin & Dominik Groos & José María, 2024. "Benchtop mesoSPIM: a next-generation open-source light-sheet microscope for cleared samples," Nature Communications, Nature, vol. 15(1), pages 1-16, December.
    2. Bani Mukhopadhyay & Cheryl Marietta & Pei-Hong Shen & Abdul Oiseni & Faridoddin Mirshahi & Maria Mazzu & Colin Hodgkinson & Eli Winkler & Qiaoping Yuan & Daniel Miranda & George Kunos & Arun J. Sanyal, 2024. "A patient-based iPSC-derived hepatocyte model of alcohol-associated cirrhosis reveals bioenergetic insights into disease pathogenesis," Nature Communications, Nature, vol. 15(1), pages 1-19, December.

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