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Single particle cryo-EM reconstruction of 52 kDa streptavidin at 3.2 Angstrom resolution

Author

Listed:
  • Xiao Fan

    (Tsinghua University
    Tsinghua University)

  • Jia Wang

    (Tsinghua University)

  • Xing Zhang

    (Tsinghua University)

  • Zi Yang

    (Tsinghua University
    Tsinghua University)

  • Jin-Can Zhang

    (Peking University)

  • Lingyun Zhao

    (Tsinghua University)

  • Hai-Lin Peng

    (Peking University)

  • Jianlin Lei

    (Tsinghua University
    Tsinghua University)

  • Hong-Wei Wang

    (Tsinghua University
    Tsinghua University)

Abstract

The fast development of single-particle cryogenic electron microscopy (cryo-EM) has made it more feasible to obtain the 3D structure of well-behaved macromolecules with a molecular weight higher than 300 kDa at ~3 Å resolution. However, it remains a challenge to obtain the high-resolution structures of molecules smaller than 200 kDa using single-particle cryo-EM. In this work, we apply the Cs-corrector-VPP-coupled cryo-EM to study the 52 kDa streptavidin (SA) protein supported on a thin layer of graphene and embedded in vitreous ice. We are able to solve both the apo-SA and biotin-bound SA structures at near-atomic resolution using single-particle cryo-EM. We demonstrate that the method has the potential to determine the structures of molecules as small as 39 kDa.

Suggested Citation

  • Xiao Fan & Jia Wang & Xing Zhang & Zi Yang & Jin-Can Zhang & Lingyun Zhao & Hai-Lin Peng & Jianlin Lei & Hong-Wei Wang, 2019. "Single particle cryo-EM reconstruction of 52 kDa streptavidin at 3.2 Angstrom resolution," Nature Communications, Nature, vol. 10(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-10368-w
    DOI: 10.1038/s41467-019-10368-w
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    Cited by:

    1. Qiang Chen & Ge Qu & Xu Li & Mingjian Feng & Fan Yang & Yanjie Li & Jincheng Li & Feifei Tong & Shiyi Song & Yujun Wang & Zhoutong Sun & Guangsheng Luo, 2023. "Active and stable alcohol dehydrogenase-assembled hydrogels via synergistic bridging of triazoles and metal ions," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    2. Jianying Zhu & Qi Zhang & Hui Zhang & Zuoqiang Shi & Mingxu Hu & Chenglong Bao, 2023. "A minority of final stacks yields superior amplitude in single-particle cryo-EM," Nature Communications, Nature, vol. 14(1), pages 1-11, December.

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