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iSuRe-Cre is a genetic tool to reliably induce and report Cre-dependent genetic modifications

Author

Listed:
  • Macarena Fernández-Chacón

    (Centro Nacional de Investigaciones Cardiovasculares (CNIC))

  • Verónica Casquero-García

    (Centro Nacional de Investigaciones Cardiovasculares (CNIC))

  • Wen Luo

    (Centro Nacional de Investigaciones Cardiovasculares (CNIC))

  • Federica Francesca Lunella

    (Centro Nacional de Investigaciones Cardiovasculares (CNIC))

  • Susana Ferreira Rocha

    (Centro Nacional de Investigaciones Cardiovasculares (CNIC))

  • Sergio Olmo-Cabrera

    (Centro Nacional de Investigaciones Cardiovasculares (CNIC))

  • Rui Benedito

    (Centro Nacional de Investigaciones Cardiovasculares (CNIC))

Abstract

Most biomedical research aimed at understanding gene function uses the Cre-Lox system, which consists of the Cre recombinase-dependent deletion of genes containing LoxP sites. This system enables conditional genetic modifications because the expression and activity of the recombinase Cre/CreERT2 can be regulated in space by tissue-specific promoters and in time by the ligand tamoxifen. Since the precise Cre-Lox recombination event is invisible, methods were developed to report Cre activity and are widely used. However, numerous studies have shown that expression of a given Cre activity reporter cannot be assumed to indicate deletion of other LoxP-flanked genes of interest. Here, we report the generation of an inducible dual reporter-Cre mouse allele, iSuRe-Cre. By significantly increasing Cre activity in reporter-expressing cells, iSuRe-Cre provides certainty that these cells have completely recombined floxed alleles. This genetic tool increases the ease, efficiency, and reliability of conditional mutagenesis and gene function analysis.

Suggested Citation

  • Macarena Fernández-Chacón & Verónica Casquero-García & Wen Luo & Federica Francesca Lunella & Susana Ferreira Rocha & Sergio Olmo-Cabrera & Rui Benedito, 2019. "iSuRe-Cre is a genetic tool to reliably induce and report Cre-dependent genetic modifications," Nature Communications, Nature, vol. 10(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-10239-4
    DOI: 10.1038/s41467-019-10239-4
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