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Nanopore blockade sensors for ultrasensitive detection of proteins in complex biological samples

Author

Listed:
  • Kyloon Chuah

    (The University of New South Wales)

  • Yanfang Wu

    (The University of New South Wales)

  • S. R. C. Vivekchand

    (The University of New South Wales)

  • Katharina Gaus

    (The University of New South Wales)

  • Peter J. Reece

    (The University of New South Wales)

  • Adam P. Micolich

    (The University of New South Wales)

  • J. Justin Gooding

    (The University of New South Wales)

Abstract

Nanopore sensors detect individual species passing through a nanoscale pore. This experimental paradigm suffers from long analysis times at low analyte concentration and non-specific signals in complex media. These limit effectiveness of nanopore sensors for quantitative analysis. Here, we address these challenges using antibody-modified magnetic nanoparticles ((anti-PSA)-MNPs) that diffuse at zero magnetic field to capture the analyte, prostate-specific antigen (PSA). The (anti-PSA)-MNPs are magnetically driven to block an array of nanopores rather than translocate through the nanopore. Specificity is obtained by modifying nanopores with anti-PSA antibodies such that PSA molecules captured by (anti-PSA)-MNPs form an immunosandwich in the nanopore. Reversing the magnetic field removes (anti-PSA)-MNPs that have not captured PSA, limiting non-specific effects. The combined features allow detecting PSA in whole blood with a 0.8 fM detection limit. Our ‘magnetic nanoparticle, nanopore blockade’ concept points towards a strategy to improving nanopore biosensors for quantitative analysis of various protein and nucleic acid species.

Suggested Citation

  • Kyloon Chuah & Yanfang Wu & S. R. C. Vivekchand & Katharina Gaus & Peter J. Reece & Adam P. Micolich & J. Justin Gooding, 2019. "Nanopore blockade sensors for ultrasensitive detection of proteins in complex biological samples," Nature Communications, Nature, vol. 10(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-10147-7
    DOI: 10.1038/s41467-019-10147-7
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    Cited by:

    1. Jianxin Yang & Tianle Pan & Zhenming Xie & Wu Yuan & Ho-Pui Ho, 2024. "In-tube micro-pyramidal silicon nanopore for inertial-kinetic sensing of single molecules," Nature Communications, Nature, vol. 15(1), pages 1-12, December.

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