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Activated Peyer′s patch B cells sample antigen directly from M cells in the subepithelial dome

Author

Listed:
  • Rathan Joy Komban

    (University of Gothenburg)

  • Anneli Strömberg

    (University of Gothenburg)

  • Adi Biram

    (The Weizmann Institute of Science)

  • Jakob Cervin

    (University of Gothenburg)

  • Cristina Lebrero-Fernández

    (University of Gothenburg)

  • Neil Mabbott

    (Edinburgh University)

  • Ulf Yrlid

    (University of Gothenburg)

  • Ziv Shulman

    (The Weizmann Institute of Science)

  • Mats Bemark

    (University of Gothenburg)

  • Nils Lycke

    (University of Gothenburg)

Abstract

The germinal center (GC) reaction in Peyer′s patches (PP) requires continuous access to antigens, but how this is achieved is not known. Here we show that activated antigen-specific CCR6+CCR1+GL7− B cells make close contact with M cells in the subepithelial dome (SED). Using in situ photoactivation analysis of antigen-specific SED B cells, we find migration of cells towards the GC. Following antigen injection into ligated intestinal loops containing PPs, 40% of antigen-specific SED B cells bind antigen within 2 h, whereas unspecifc cells do not, indicating B cell-receptor involvment. Antigen-loading is not observed in M cell-deficient mice, but is unperturbed in mice depleted of classical dendritic cells (DC). Thus, we report a M cell-B cell antigen-specific transporting pathway in PP that is independent of DC. We propose that this antigen transporting pathway has a critical role in gut IgA responses, and should be taken into account when developing mucosal vaccines.

Suggested Citation

  • Rathan Joy Komban & Anneli Strömberg & Adi Biram & Jakob Cervin & Cristina Lebrero-Fernández & Neil Mabbott & Ulf Yrlid & Ziv Shulman & Mats Bemark & Nils Lycke, 2019. "Activated Peyer′s patch B cells sample antigen directly from M cells in the subepithelial dome," Nature Communications, Nature, vol. 10(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-10144-w
    DOI: 10.1038/s41467-019-10144-w
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    Cited by:

    1. Lucia Montorsi & Michael J. Pitcher & Yuan Zhao & Chiara Dionisi & Alicia Demonti & Thomas J. Tull & Pawan Dhami & Richard J. Ellis & Cynthia Bishop & Jeremy D. Sanderson & Sahil Jain & David D’Cruz &, 2024. "Double-negative B cells and DNASE1L3 colocalise with microbiota in gut-associated lymphoid tissue," Nature Communications, Nature, vol. 15(1), pages 1-17, December.

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