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Dynamic assembly of ribbon synapses and circuit maintenance in a vertebrate sensory system

Author

Listed:
  • Haruhisa Okawa

    (University of Washington)

  • Wan-Qing Yu

    (University of Washington)

  • Ulf Matti

    (Saarland University)

  • Karin Schwarz

    (Saarland University)

  • Benjamin Odermatt

    (University of Bonn)

  • Haining Zhong

    (Oregon Health and Science University)

  • Yoshihiko Tsukamoto

    (Hyogo College of Medicine)

  • Leon Lagnado

    (University of Sussex)

  • Fred Rieke

    (University of Washington)

  • Frank Schmitz

    (Saarland University)

  • Rachel O. L. Wong

    (University of Washington)

Abstract

Ribbon synapses transmit information in sensory systems, but their development is not well understood. To test the hypothesis that ribbon assembly stabilizes nascent synapses, we performed simultaneous time-lapse imaging of fluorescently-tagged ribbons in retinal cone bipolar cells (BCs) and postsynaptic densities (PSD95-FP) of retinal ganglion cells (RGCs). Ribbons and PSD95-FP clusters were more stable when these components colocalized at synapses. However, synapse density on ON-alpha RGCs was unchanged in mice lacking ribbons (ribeye knockout). Wildtype BCs make both ribbon-containing and ribbon-free synapses with these GCs even at maturity. Ribbon assembly and cone BC-RGC synapse maintenance are thus regulated independently. Despite the absence of synaptic ribbons, RGCs continued to respond robustly to light stimuli, although quantitative examination of the responses revealed reduced frequency and contrast sensitivity.

Suggested Citation

  • Haruhisa Okawa & Wan-Qing Yu & Ulf Matti & Karin Schwarz & Benjamin Odermatt & Haining Zhong & Yoshihiko Tsukamoto & Leon Lagnado & Fred Rieke & Frank Schmitz & Rachel O. L. Wong, 2019. "Dynamic assembly of ribbon synapses and circuit maintenance in a vertebrate sensory system," Nature Communications, Nature, vol. 10(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-10123-1
    DOI: 10.1038/s41467-019-10123-1
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