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Differential human antibody repertoires following Zika infection and the implications for serodiagnostics and disease outcome

Author

Listed:
  • Supriya Ravichandran

    (Center for Biologics Evaluation and Research (CBER), FDA)

  • Megan Hahn

    (Center for Biologics Evaluation and Research (CBER), FDA)

  • Pablo F. Belaunzarán-Zamudio

    (Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán)

  • José Ramos-Castañeda

    (Instituto Nacional de Salud Publica)

  • Gabriel Nájera-Cancino

    (Hospital Regional de Alta Especialidad Ciudad Salud)

  • Sandra Caballero-Sosa

    (Instituto de Seguridad y Servicios Sociales de los Trabajadores del Estado)

  • Karla R. Navarro-Fuentes

    (Instituto Mexicano del Seguro Social)

  • Guillermo Ruiz-Palacios

    (Comisión Coordinadora de los Institutos Nacionales de Salud y Hospitales de Alta Especialidad, Ministry of Health)

  • Hana Golding

    (Center for Biologics Evaluation and Research (CBER), FDA)

  • John H. Beigel

    (Frederick National Laboratory for Cancer Research
    National Institutes of Health)

  • Surender Khurana

    (Center for Biologics Evaluation and Research (CBER), FDA)

Abstract

Zika virus (ZIKV) outbreak in Americas led to extensive efforts to develop vaccines and ZIKV-specific diagnostics. In the current study, we use whole genome phage display library spanning the entire ZIKV genome (ZIKV-GFPDL) for in-depth immune profiling of IgG and IgM antibody repertoires in serum and urine longitudinal samples from individuals acutely infected with ZIKV. We observe a very diverse IgM immune repertoire encompassing the entire ZIKV polyprotein on day 0 in both serum and urine. ZIKV-specific IgG antibodies increase 10-fold between day 0 and day 7 in serum, but not in urine; these are highly focused on prM/E, NS1 and NS2B. Differential antibody affinity maturation is observed against ZIKV structural E protein compared with nonstructural protein NS1. Serum antibody affinity to ZIKV-E protein inversely correlates with ZIKV disease symptoms. Our study provides insight into unlinked evolution of immune response to ZIKV infection and identified unique targets for ZIKV serodiagnostics.

Suggested Citation

  • Supriya Ravichandran & Megan Hahn & Pablo F. Belaunzarán-Zamudio & José Ramos-Castañeda & Gabriel Nájera-Cancino & Sandra Caballero-Sosa & Karla R. Navarro-Fuentes & Guillermo Ruiz-Palacios & Hana Gol, 2019. "Differential human antibody repertoires following Zika infection and the implications for serodiagnostics and disease outcome," Nature Communications, Nature, vol. 10(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-09914-3
    DOI: 10.1038/s41467-019-09914-3
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